We narrowed to 566 results for: FIE

1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...

2. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...

3. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   Published

   March 31, 2015, 4:08 p.m.

   ...bringing and effecting solutions to both known and unidentified issues. There are many good resources on the...

4. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...Dissolve in dH2O and sterile filter unless otherwise specified. The above table lists some antibiotics commonly...

5. Choosing a Good Mentor for Scientists

   Type

   Blog Post

   Published

   Jan. 16, 2014, 2:56 p.m.

   ... By contrast, as a postdoc, you should choose a field that you may want to work on in your future career...

6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...vectors are viruses that have been genetically modified to either limit or completely eliminate their ...

7. Meet the New Addgene Board Members!

   Type

   Blog Post

   Published

   April 28, 2020, 1:14 p.m.

   ...solutions to crises like those and ended up in the field of gene therapy.” He’s also worked on vaccines throughout...

8. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   Published

   June 27, 2018, 1:16 p.m.

   ...Reverse transcriptases are critical tools for many fields of biology and enable the conversion of RNA templates...

9. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

   Type

   Blog Post

   Published

   April 12, 2018, 1:02 p.m.

   ... learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing...

10. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    Published

    Feb. 14, 2018, 2:36 p.m.

    ...and techniques are not enough to move the CRISPR field forward. To do that, we need to learn much more ...

11. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    Published

    Dec. 21, 2017, 2:06 p.m.

    ...catalog every variant of a feature. Instead, we identified common variants, and then crafted a detection...

12. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions...

13. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

    Published

    March 28, 2022, 4:30 p.m.

    ..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...

14. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ... proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection...

15. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...pseudovirus neutralization assay. Neutralization was quantified in terms of luminescence. Image from Garcia-Beltran...

16. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...article.) Modifying AraC to create BLADE The team modified AraC to create BLADE by replacing its dimerization...

17. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...blue-shifting more than others. During this process, they identified mWasabi, a bright, monomeric green fluorescent...

18. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    Published

    Jan. 22, 2019, 2:41 p.m.

    ...every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine...

19. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...unusual job openings, although they seemed too far afield at first and I let them pass by. But I didn’t forget...

20. Plasmids 101: Origin of Replication

    Type

    Blog Post

    Published

    Nov. 11, 2020, 2:23 p.m.

    ...die off. Have any questions? Need something clarified? If so please, post in the comments.  References...