We narrowed to 570 results for: FIE

...at 37 °C. These short DNA fragments can then be purified for subsequent library preparation and high-throughput...

...Aligning sequences with Snapgene    Once we’ve identified the features within a plasmid, we next verify...

...piece of advice for those looking to break into the field of science communications-- Be able to talk with...

... of GeoCas9 by the Doudna lab has opened up the field of thermophilic bacteria to CRISPR/Cas9 genome editing...

...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...

...bringing and effecting solutions to both known and unidentified issues. There are many good resources on the...

...Dissolve in dH2O and sterile filter unless otherwise specified. The above table lists some antibiotics commonly...

... By contrast, as a postdoc, you should choose a field that you may want to work on in your future career...

...tracing, among other applications. Cells can be identified using fluorescence, like in the pMuSIC library...

...career in science communication, an established field of inquiry with which I was not yet familiar. The...

..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...

... proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection...

...pseudovirus neutralization assay. Neutralization was quantified in terms of luminescence. Image from Garcia-Beltran...

...article.) Modifying AraC to create BLADE The team modified AraC to create BLADE by replacing its dimerization...

...vectors are viruses that have been genetically modified to either limit or completely eliminate their ...

...solutions to crises like those and ended up in the field of gene therapy.” He’s also worked on vaccines throughout...

...uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions...

...Reverse transcriptases are critical tools for many fields of biology and enable the conversion of RNA templates...

... learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing...

...and techniques are not enough to move the CRISPR field forward. To do that, we need to learn much more ...