We narrowed to 468 results for: SEC

1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range of bacteria...

2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...re-cutting but does not disrupt gene expression. (See section below.) For guide design, we decided to adapt the...

3. Academic vs. Industry Postdocs

   Type

   Blog Post

   Published

   Nov. 13, 2018, 1:22 p.m.

   ...and the jobs are mixed into the general careers section of their web site. Each position is for a specific...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...required for proper packaging of AAV, but also form secondary structures that are prone to deletion from the...

5. Of Myc and Men

   Type

   Blog Post

   Published

   Jan. 19, 2023, 2:15 p.m.

   ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   Published

   July 2, 2020, 12:40 p.m.

   ...organization of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction...

7. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   Published

   Aug. 29, 2019, 12:50 p.m.

   ...ensures you aren’t scrambling to tack up your poster seconds before the session begins. Plus, you’ll have extra...

8. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...conference Practice your “elevator pitch,” the 30 - 60 second blurb you will use to introduce yourself and what...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...vectors system, Manuel Gonçalves' lab found in a second paper published in Nature Methods the same year...

10. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ... which ultimately led to the engineering of the second type of these GEVIs: a FP-fused rhodopsin. FP-fusion...

11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...throughout the text or see the “Additional Resources” section at the end of the post! Speed: Your grant deadline...

12. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

13. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...should be able to draw perpendicular lines that intersect your populations. If you see a diagonal population...

14. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate endogenously...

15. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...could do all these tasks before I was a manager! Second, my direct report was not engaged because I only...

16. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...desirable properties, either initially, or using secondary biological screens. For example, for cancer researchers...

17. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ... Please leave us your thoughts in the comments section below. Michael G. Lemieux is a Scientist at Addgene...

18. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...mAmetrine1.2). However, for most FPs it is a few seconds to a few minutes. Photostability can be affected...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ... plants. We are really plugging into the "stuff sector" if you will, making chemicals and materials, and...

20. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ... such as the chicken beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by...