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Showing: 381 - 400 of 704 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and ... on your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel ... and 2 in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...consider (see our “technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing... Splice variants Check literature Posttranslational modifications Check literature Highly...

  2. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our ...

  4. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...time we have saved researchers. In a recent blog post, we looked at how long it takes to make one plasmid...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... or detail. By the end of 2023, we had over 900 posts, which were read by researchers all over the world...mean all over (Figure 3). We continue to add new posts every week, and we’ve made our popular blog series...

  7. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...visualization methods. Stay tuned for a future blog post to learn more about these techniques!  Direct vs...effective and reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

  8. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...relationships you develop will lead! Read our blog post about fostering communication in remote collaborations...Blog Check out Our Career Topic Page Read Other Posts in the Transferable Skills Guide Developing Transferable...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create...collaborative environment. Think of student groups, PostDoc associations and specific projects where you share...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...proximity to the region to be edited. In this blog post, we will cover three ways to circumvent this limitation...and Mary Gearing contributed to the update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler...

  11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful...between loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different...markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in high concentrations...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Out Our Networking for Scientists Blog Posts Read Our Career Posts Read Joanne's Management for Scientist's...frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice giving ...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...different types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less ...

  14. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...Another factor to consider is the age of your culture post thaw. In general, the higher the passage number ...produce the virus, titer the virus (read this blog post for tips on titering your lentiviral prep!) and ...

  16. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...January 28. (Article). Read More: Other CRISPR Blog Posts ...

  17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... major ones in Addgene’s repository later in the post. Advantages of prime editing Less constrained by...treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration of...plasmids! Find plasmids from David Liu's lab! This post was originally written by Jennifer Tsang in October...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...parallel assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art...
Showing: 381 - 400 of 704 results