We narrowed to 695 results for: prot

...nuclease that is active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer...CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9...without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes...resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9...

...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in...

...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian ... they use; even if two species encode the same protein, their gene sequence may have slight or large variations...resembles that of the host organism, it may express protein more efficiently. To classify gene sequences as...

...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...systematically explore variations in the spike protein, teaching us more about viral evolution and antibody... Pooled Libraries CRISPR Library Amplification Protocol ...

...system was enhanced to allow searches for a perfect protein sequence match even when the DNA sequence has changed... inaccurately. The best example is fluorescent proteins, which often come in closely related versions ..., we augment the database with new fluorescent protein variants. The future of DNA feature identification...Collections”, which are shareable databases of DNA and protein sequences, the next step is to enhance Collections...

...colleagues began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified...calcium binding and developed a version of the protein with as few calcium-binding sites as possible for...transcription factor cAMP-responsive element-binding protein (CREB) in living cells. “Now we are making this...Eindhoven has similar interest in FRET-based sensor proteins, particularly for the intracellular imaging of...

...organized allowing you to upload anything from protocols to pictures of gels or even sketches to your notebook...but the NEB Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app...Bioinformatic Analyses Use Apps to Share Your Lab Protocols Check Out How Addgene Develops Lab Management ...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular Biology...

...nucleofection, which introduces functional Cas9 protein and the targeting guide RNA into cells by electroporation...extremely high frequencies of HDR. The detailed protocol for nucleofection as well as the sequences of ...is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....

...performance of antibodies, because of effects of pH on protein stability (Murphy et al., 2013). However, the pH...Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

...gramstain.html Smith, A. & Hussey, M. Gram Stain Protocols. American Society for Microbiology (2005). https...5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol-2886.pdf Steward, K. Gram Positive vs Gram Negative...Hans-Christian-Gram.pdf Smith, A. & Hussey, M. Gram Stain Protocols. American Society for Microbiology (2005). https...

...standard lentivirus production protocol here and you can find more tips and protocols for working with lentivirus...researcher can complete an entire lentiviral production protocol only to find that the low titer of her prep makes...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

...that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases involved with...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

...helping us establish consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from...technical service (details about virus production protocols and quality control can be found on Addgene’s ...and Office Teams develop shipping systems and protocols for viral vectors to reach scientists around the...Beginners Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector Pages Find Virus for Your...

...readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The most obvious...includes tension monitors associated with specific proteins of interest such as actinin. When tension is high....2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service: Biosensor AAV Viral...Epigenetics with a FRET Based Biosensor Fluorescent Proteins 101: Fluorescent Biosensors ...

...indel generation for two PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing...3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly higher mutation...mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How many loci can...gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted genomic sites separated...

...Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous... plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide library...spot to fuse the activators instead of at the protein’s C terminus. With that new design in place, Konermann... one time. Weissman’s group has made standard protocols available and they are working to further optimize...

...possibly inducing the secretion of growth promoting proteins, such as human growth factor, vascular endothelial...strong in this one. After all, the system built to protect us now seems to attack us by releasing mitogenic...microenvironment itself. They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment...exosomes expressed specific integrins (cell-adhesion proteins) targeting different organs. This means that exosomes...

...mammalian cells, especially when fused to effector proteins for gene activation or gene editing. The more ...various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical...delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own mRNA to facilitate...RNA motif. These engineered pegRNAs (epegRNAs) protect the RNA from degradation by cellular exonucleases...

...create an almost limitless number of DNA binding proteins with Cas9 guided by different promoters. The challenge... genetic circuits is getting enough regulatory proteins that don’t interfere with each other. CRISPR is...much easier it is to work with than regulatory proteins. There are still things we haven’t solved that...Nothing too much more sophisticated. There are also proteins, like antibodies. But we are really just using...

...composed of a tightly-bound invariant group of proteins, whereas the more loosely bound co-coregulators...explain how more than 300 different coactivator proteins individually contribute to cell regulation in ...coherent manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...