We narrowed to 839 results for: LEA
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TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m.... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...
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How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m.... interview. Each interview is an opportunity to learn something and then apply it to the next interview... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find in this ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... your research! If they do (or if they don't!), please consider sharing your experience with other researchers... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....science happens in a vacuum! Munro and Pelham learned of these new antibodies and identified Myc1-9E10... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... their minds, but I think many of the students learned something. Based on the initial guidelines I received... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m.... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m..... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m.... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PMC4737176. Additional Resources on the Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless: genetic... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....structural understanding of your protein that can lead to novel insights about its biology and its behavior... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....slightly more expensive gradient gel, which will allow clear separation of proteins of different sizes. Table...