We narrowed to 507 results for: CIT

1. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability when using...

2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...control of signaling pathways. This is particularly exciting because using light to control signaling provides...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...engineered cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...the leap into management, the journey can be as exciting as it is challenging. How do you prepare for...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts bigger...

6. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...specifically in their favorite neurons. This specificity is feasible partially because of viruses—keep...

7. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on which protocols would...

8. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ... in BioRender or use appropriately licensed and cited figures from papers or image sharing repositories...

9. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...genes are assayed for their role in a particular capacity. Drug screens, on the other hand, are often performed...

10. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...

11. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

12. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ..., please fill out this form and we’ll add your citation to the plasmid page on our website. Contributing...

13. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...SynBio arena this year with a fresh team of twenty excited undergrads. Team IISER-B’s idea for the iGEM 2019...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...Spring Harbor in March. A number of groups were excited and asked for the protocol. Since the Addgene page...

15. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...the repository, MoClo and GreenGate, and we are excited to see how this exchange of genetic tools facilitates...

16. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

18. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas...

19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard...

20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...CRISPR RNAs to improve Gene-Editing Activity and Specificity. JACS 144, 12584-12594 (2022). https://doi/pdf...