We narrowed to 504 results for: CIT

1. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

2. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...specifically in their favorite neurons. This specificity is feasible partially because of viruses—keep...

3. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on which protocols would...

4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts bigger...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...the leap into management, the journey can be as exciting as it is challenging. How do you prepare for...

6. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ... in BioRender or use appropriately licensed and cited figures from papers or image sharing repositories...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...genes are assayed for their role in a particular capacity. Drug screens, on the other hand, are often performed...

8. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...SynBio arena this year with a fresh team of twenty excited undergrads. Team IISER-B’s idea for the iGEM 2019...

9. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...control of signaling pathways. This is particularly exciting because using light to control signaling provides...

10. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ...create a program that would capitalize on the excitement found through experiential learning. “Students...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...Spring Harbor in March. A number of groups were excited and asked for the protocol. Since the Addgene page...

12. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...the repository, MoClo and GreenGate, and we are excited to see how this exchange of genetic tools facilitates...

13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

14. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas...

15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard...

17. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ... scientific communication needs. The most often-cited reasons researchers might not post a preprint are...

18. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ... Holmes Several labs have recently shared some exciting plasmid tools to optimize prime editing systems...

19. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...when used in structural studies and reduces immunogenicity when used in vivo.     Of course, the lack ...

20. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...finally dive deep into generating the data you’re excited about. When we were closing in on the one million...