Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 749 results for: SOM

Showing: 401 - 420 of 749 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...Based on personal preference, some people like to make stricter gates while some like to make looser ones ... your cells on a flow plot, you might have to do some testing or ask experienced colleagues.   ...

  2. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...knock-ins Are you introducing a large insertion, something 20 base pairs or bigger? Then screening by PCR... pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment ...effects with Sanger sequencing, provided you have some idea of where in the genome you might expect off...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...replication. However, this approach could permit some viral protein production, so researchers are also...Cas9 cut site, leading Wang et al. to conclude that some Cas9-derived indels may not ablate viral function...would open up many new therapeutic possibilities for some of the world’s most common diseases. References...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...different naturally occurring strains of E. coli, some of which are deadly to humans. The majority of all...for growth rpsL Mutation in subunit S12 of 30S ribosome Confers resistance to streptomycin Tn10   Confers...Additionally, Table 2 provides a quick reference for some of the popular strains, their genotypes, and their...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Drosophila or development studies, you may hear some transcription factor and promoter pairs referred...behave very similarly?” The simple answer is that sometimes it is necessary or desirable to control or look...Conclusion This post contains only a brief overview of some of the most common repressible promoter systems,...

  6. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...enables the design of primer pairs that PCR amplify in some states but not others. Because samples of many registers...output plasmid (single copy, bacterial artificial chromosome backbone) contains the register. Depending on... that, depending on the context and application, some inducible recombinase systems may work faster or...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...offspring. Although powerful, this technology is cumbersome to use, not that efficient, often takes more ...must beware that not all gRNAs are created equal. Some work better than others. There are a variety of ...other gRNA design tools available and you can get some additional advice from John Doench’s gRNA design...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on random integration...there is no selection, you will need to screen somewhere between one hundred and one thousand clones to...boost efficiency over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-joining activity...those containing one or more mismatches. However, some ligases can ligate mismatches to a significant degree...pyrimidine:purine mismatches. Helix stability also plays some role, and mismatches with more hydrogen bonds are...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... themselves from one location and then insert somewhere else (Fig. 2). Class II TEs have LTRs on both ...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take a ...and cell therapy, and have potential to overcome some of the limitations of viruses. TEs have a large ...

  11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...swarming around S. aureus and eating the bacteria. Some of the many questions scientists have been able ...used in your experiments, experience has shown that some are more reliable and more efficient at tracking... Tips on Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent Proteins...

  12. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...therapies themselves can be made more effective. Some important factors researchers are beginning to focus...broadly activate the immune response against tumors. Some chemotherapeutic agents induce immunogenic tumor...deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death receptor...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...approaches are commonly used to produce KO cell lines, some of which are commercially available. Various proteomic...multiple techniques. For example, we have found that some antibodies perform well in a PFA-saponin fixation...). The approach maps onto the five pillars, with some specific recommendations for how to use these in...

  14. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping...

  15. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...on the basics of viral vectors, applications, and some new resources available from Addgene. There’s a ...vectors In addition to “going viral,” we also have some other exciting news. Last week, we launched our ...

  16. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...after all a place that encourages people to take some time out every once in a while – just read Melina... people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, of course...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...available online if you prefer the DIY method. As some proteins aggregate at 95 °C, you may want to check...preserve sample, check the ingredients before using, as some contain Tween-20, which can strip proteins from ...non-specific binding; lot-to-lot variability Some recombinant antibodies Not always available; not...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...received a festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them... to everyone who decked their lab and brought us some holiday cheer!   ...

  19. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...export license application for rabies materials! Some of the materials in our repository, including rabies...license for rabies materials may be required for some countries and may not be required for others. You...

  20. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ... about their career paths, future goals, and get some useful career advice.  Our first video, featuring...and interesting content. Wish we had a video on something that you don’t see here? Give us suggestions in...
Showing: 401 - 420 of 749 results