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Showing: 401 - 420 of 430 results

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Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

Published

June 20, 2024, 1:15 p.m.

...machinery for elongation and termination. These types of plasmids tend to be high copy number. Here, we...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

Published

Sept. 13, 2018, 1:55 p.m.

...screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue), which target...
Tips for CRISPR Gene Editing in Mice

Type

Blog Post

Published

June 28, 2016, 10:59 a.m.

...determine the pros and cons of using many different types of gRNA design software. When you are happy with...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

Published

Oct. 27, 2015, 3 p.m.

...replication machinery of Kaposi’s sarcoma-associated herpesvirus. References 1. Snapp, Erik. "Design and...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

Published

Oct. 1, 2015, 2:30 p.m.

...of c-fos amongst animals that receive different types of stimulation may help to refine and optimize your...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

Published

Feb. 10, 2015, 3:06 p.m.

...be discussed later). In addition to the basic genotypes outlined below, certain specialized strains are...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

Published

Sept. 5, 2017, 1:58 p.m.

...stranded breaks (DSBs) into genomic DNA in many cell types and often results in biallelic modifications. Most...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

Published

Feb. 22, 2017, 3:30 p.m.

...Disadvantages of this method include favoring of certain types of mutations, difficulty controlling the rate of...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

Published

Oct. 11, 2016, 2:30 p.m.

....2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation. Nature 532:293–293 . https://doi.org...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

Published

March 27, 2018, 1:32 p.m.

...of choice for gene delivery to a variety of cell types, now you know that AAVs can provide a powerful boost...
Five Popular Model Organisms

Type

Blog Post

Published

April 11, 2019, 12:39 p.m.

... diseases. Diseases in mice show many similar phenotypes to human diseases and have successfully helped...
Plasmids 101: Biotinylation

Type

Blog Post

Published

Nov. 15, 2018, 1:50 p.m.

...using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an oligonucleotide...
Five Popular Model Organisms, Part 2

Type

Blog Post

Published

Sept. 29, 2020, 1:15 p.m.

...Pisula, W. (2020). The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

Published

June 3, 2021, 1:15 p.m.

...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

Published

Jan. 26, 2016, 3:30 p.m.

...methods, they each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

Published

Nov. 25, 2015, 4 p.m.

... 25590106. Paula Stephan’s Book - How Economics Shapes Science The Future of Research Website Additional...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

Published

Nov. 7, 2014, 2:56 p.m.

...reference for some of the popular strains, their genotypes, and their primary use in the lab. These strains...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

Published

May 18, 2018, 7:32 p.m.

...precisely. You can find a great discussion of different types of replication in Nosek and Errington 2017 and Schmmidt...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

Published

June 24, 2020, 5:45 p.m.

... targeted delivery into hard-to-manipulate cell types. They have also fused LSD1 to x-Cas9(3.7). x-Cas9...

Showing: 401 - 420 of 430 results

Results per page:

Plasmids 101: Stringent Regulation of Replication

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Tips for CRISPR Gene Editing in Mice

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: E. coli Strains for Protein Expression

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Deep Mutational Scanning with One Pot Saturation Mutagenesis

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Fluorescent Proteins 101: When GFP lets you down

Adeno-associated Viruses (AAVs) for Genome Editing

Five Popular Model Organisms

Plasmids 101: Biotinylation

Five Popular Model Organisms, Part 2

Antibodies 101: Single Chain Fragment Variables (scFvs)

Treating Muscular Dystrophy with CRISPR Gene Editing

Thoughts on the Future of Research 2015 Boston Symposium

Plasmids 101: Common Lab E. coli Strains

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

CRISPR 101: Epigenetics and Editing the Epigenome

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