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We narrowed to 777 results for: REP

Showing: 421 - 440 of 777 results

  1. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...majority of them remain uncharacterized. This represents a considerable barrier to furthering research...in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80% (over 800...) and EGFR (Plasmid 116731: pHAGE-EGFR), are represented in this collection along with hundreds of less...

  2. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....contributors, including 61 fluorescent probes, representing imaging from 47 different tissues. Researchers...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

  3. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

  4. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...is the 21st century and all. But while the auto-reply telling you your message was received is a nice ... on hold, let alone waiting multiple days for a reply to an email. You need answers: the right ones, quickly...everyone involved. We’re adding self service to the repertoire of tools you can use to get support for our resources...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...monitoring submissions (usually a Human Resources representative). Pro tip: if there is no place to upload a...

  6. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

  7. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...booth about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against bacteriophages...for their ability to devise new strategies to reproduce within a host cell. A few virus particles have...

  9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...error rates compared to other RTs. MarathonRT represents a major step forward in facilitating techniques.... Nature Methods. 2018. PMID: 29735997 bioRxiv preprint posted July 4, 2017 A new method to identify...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

  11. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  12. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

  17. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  18. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...flexible multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds...single guide RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...

  20. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...might like to store about FPs than is currently represented at fpvis (complete spectral information, accession...rapidly growing, with your help!), I believe this represents one of the largest available online collections...analysis tools, and a better way to store and represent within-study photobleaching comparisons.  We very...
Showing: 421 - 440 of 777 results