We narrowed to 791 results for: REP

...grant proposals or to demonstrate impact in annual reports. While the raw data is, of course, interesting ...data, including total requests, total countries represented by requestors, year of first reagent sent, year...and the source code is available in the Github repository (you can find the link in the upper right hand...

... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...

...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...being part of our growing community! Our plasmid repository succeeds because of our amazing community. The...

...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those are the biggest...

...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already...

...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science ...that too few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get... finding ways to provide new types of career preparedness training for their scientists. I have worked...

...types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh ...un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks DNA ...breaks and may better facilitate homology directed repair. You can find all of these variants, as well as...

...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base in its ...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...Engineering in March is fused to a translational repressor. In principle, however, designer PUF proteins can...

...deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting...

...plasmids” from last March were quite popular and represent the diversity of tools we share on behalf of scientists...and guest blogger Erik Snapp breaks down how to prepare a chalk talk for an academic job interview including... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

...majority of them remain uncharacterized. This represents a considerable barrier to furthering research...in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80% (over 800...) and EGFR (Plasmid 116731: pHAGE-EGFR), are represented in this collection along with hundreds of less...

...conduct a two hour long interview. I anxiously prepared for the call. The length of this interview was...was to do for my time at Addgene. This training prepared me for the independent work on the QC team. I ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....contributors, including 61 fluorescent probes, representing imaging from 47 different tissues. Researchers...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

...is the 21st century and all. But while the auto-reply telling you your message was received is a nice ... on hold, let alone waiting multiple days for a reply to an email. You need answers: the right ones, quickly...everyone involved. We’re adding self service to the repertoire of tools you can use to get support for our resources...

...for a successful application. It takes time to prepare an excellent resume…it is worth it. Read here to... to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and ...monitoring submissions (usually a Human Resources representative). Pro tip: if there is no place to upload a...

...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

...booth about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...

...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against bacteriophages...for their ability to devise new strategies to reproduce within a host cell. A few virus particles have...

...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...