We narrowed to 809 results for: org

1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...

2. New and Upcoming Viral Vectors - June 2019

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   Blog Post

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   June 18, 2019, 1:15 p.m.

   ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...

3. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

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   Aug. 22, 2023, 1 p.m.

   ...something. Offer to help out wherever you can and don’t forget to express your gratitude. Be the mentee that YOU... YOU would like to train! And, of course, don’t forget to have fun! Visiting is an amazing career opportunity...

4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

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   March 30, 2023, 1:15 p.m.

   ...available, just ask an Addgene scientist at help@addgene.org! References and Resources  References Joung ...Lentiviral Transduction Additional Resources on Addgene.org Plasmid Pooled Libraries Pooled library amplification...

5. Creating accessible biology activities in schools with BioBits

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   Blog Post

   Published

   Aug. 2, 2018, 12:56 p.m.

   ...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

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   Sept. 19, 2017, 1:20 p.m.

   ...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...

7. Pushing the Limits of DNA Assembly

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   Blog Post

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   Sept. 6, 2017, 1:01 p.m.

   ...enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in...Plasmids by Colony PCR Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs...

8. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

9. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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   Blog Post

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   Aug. 28, 2014, 3:34 p.m.

   ...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...visualize the DNA under ultraviolet (UV) light. Don’t forget to add loading buffer to your digest reactions ...

10. Finding and Joining Your Dream Lab

    Type

    Blog Post

    Published

    April 8, 2014, 2:50 p.m.

    ...The experiments must fit with their interests.” George Church selects new hires initially based on those... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...

11. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...check out our updated CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational...import of newly synthesized (green) protein and organelles with older (yellow to orange) protein. Image ...favorite plasmid technologies? Email us at blog@addgene.org  and let us know what plasmids you would like...

12. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    Published

    May 28, 2020, 1:15 p.m.

    ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...

13. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...Molecular Biology. Humana Press, pp 331–338 https://doi.org/10.1007/978-1-59745-181-9_23 Additional resources...about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids from Ginkgo Bioworks  Find...

14. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment...

15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

16. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

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    June 25, 2019, 12:54 p.m.

    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

17. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...

18. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...www.ncbi.nlm.nih.gov/books/NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's...

19. Viral Vectors 101: Biosensors

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    Blog Post

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    July 6, 2023, 1 p.m.

    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

20. Viral Vectors 101: Types of viruses

    Type

    Blog Post

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    June 6, 2023, 1:15 p.m.

    ...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...10):2073. DOI: 10.3390/v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene...