...27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were even ...into M phase oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that...articles and commentaries) pop up on Twitter and Facebook, shared by biologists and non-biologists alike...

...transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under the control of...needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a constitutive promoter...production titers decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans...

...quicker to pick up new languages!) Jargon has a place in science communication, but the more you use, ...of detail Again, this is an obvious one on the surface, but it’s quite easy to add in “necessary” detail...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

...talked with Voigt about this collision that’s taking place between CRISPR technology and synthetic biology,.... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental... Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 ...

...the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

...and recurrent infections along with a mild or exacerbated inflammatory response which leads to nearly 25%...inhibits a potassium channel, causing release of acetylcholine. Given its unique molecular biology and previous...pathophysiological role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is...

...experimenter picks up a mouse from the home cage and places it into the experimental setup, the mouse becomes...Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller and may...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ... like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare 5-6...arrangements with those you live with to have a quiet place to take your video call.  Expect technical difficulties...

...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

...indicated by exclamation marks. Error points include places where DNA is being created, such as PCR amplification...request the lab provide a new, sequence-verified replacement for the plasmid, and the full QC process is started... started over again after receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s ...