We narrowed to 504 results for: CIT

1. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...which you can also deposit with Addgene!) We’re excited to help the Doench lab share this new tool and ...

2. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...and base editing applications, with high editing capacity in both contexts for NRN PAMs and to a lesser ...

3. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ... virus. Our original proof-of-concept work was exciting because it enabled gene circuit-based sensors ...

4. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...

5. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...FLEx switch takes advantage of the orientation specificity of the site-specific recombinases (SSRs) Cre ...

6. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ...computer model. When I first heard about it, I got excited from a computer scientist perspective. But, the...

7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   Published

   June 5, 2014, 3:32 p.m.

   ...accelerator program who want us to succeed. We’re excited to see what the next few months have in store for...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...Drosophila can be used to push your research in new and exciting directions. Drosophila are very easy to manipulate...

9. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...mismatch tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs...

10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ... locus. The two methods had similar rates of specificity for the DNA sequence targeted in the experiment...

11. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...example of the emission spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow...

12. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

13. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...might help formulate some hypotheses. They are excited to see other researchers from a range of fields...

14. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

16. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ...using different Type IIS restriction enzyme specificities at each step. Factors such as enzyme efficiency...

17. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...residues. For Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2]....

19. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...

20. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying...