We narrowed to 791 results for: REP

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...most common expression system within the Addgene repository is mammalian, but the largest source of unique...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...error rates compared to other RTs. MarathonRT represents a major step forward in facilitating techniques.... Nature Methods. 2018. PMID: 29735997 bioRxiv preprint posted July 4, 2017 A new method to identify...

... This allowed us to create pseudoviruses that represent lineages and variants that are actually transmitting... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...pseudovirus was similar to that of pseudoviruses representing a bat-derived coronavirus and SARS-CoV. These...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

...flexible multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds...single guide RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...might like to store about FPs than is currently represented at fpvis (complete spectral information, accession...rapidly growing, with your help!), I believe this represents one of the largest available online collections...analysis tools, and a better way to store and represent within-study photobleaching comparisons.  We very...

...plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided, different tRNAs...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by...

... organisms formally classified as fungi. They reproduce by either fission or budding, which is the primary...joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR). HR is...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer...

...sequencing. The sequencing results covered TFs representing 92% of the MORF library, with an average of ...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...Documentation of the network that initiates biological reprogramming is a valuable reference if you are looking to...

...Here are a few things to consider when you're preparing your resume for a non-academic position: What ...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,..., but the earlier you ask yourself, the better prepared you are for your next step. Here are some great...

...each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols for educational...and vector type, while a search scoped to viral preps shows filters for promoter and serotype, among others...filter value. For example, there is only one viral prep filter for promoter “Syn”, that, when selected, ...

... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from...and AAV plasmids as well as ready-to-use viral preparations at Addgene. For more information on viral vectors... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...