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We narrowed to 777 results for: REP

Showing: 441 - 460 of 777 results

  1. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided, different tRNAs...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

  2. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by...

  3. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

  4. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  5. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ... organisms formally classified as fungi. They reproduce by either fission or budding, which is the primary...joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR). HR is...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...sequencing. The sequencing results covered TFs representing 92% of the MORF library, with an average of ...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...Documentation of the network that initiates biological reprogramming is a valuable reference if you are looking to...

  8. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...Here are a few things to consider when you're preparing your resume for a non-academic position: What ...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,..., but the earlier you ask yourself, the better prepared you are for your next step. Here are some great...

  9. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...persuading an employer that your past work has prepared you to contribute at a high level to their team...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...form? Training in science communication should prepare you to ask and answer these questions for a diverse...

  10. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from...and AAV plasmids as well as ready-to-use viral preparations at Addgene. For more information on viral vectors... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...bacteria resistant to both spectinomycin and streptomycin, but could also be used for studying epistatic...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...

  12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...will see two bands: one representing your vector backbone and the other representing the insert. If the plasmid...using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using colony...

  13. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...uninfected host cell culture). This dilution step represents the major difference between PACE and PANCE: ...that change C to T, G to A, A to G, and T to C, represent about two-thirds of known pathogenic point mutations...

  14. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would...a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, an outreach scientist from...The talk highlighted the importance of reagent repositories and the myriad facilities offered by them which...

  15. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols for educational...and vector type, while a search scoped to viral preps shows filters for promoter and serotype, among others...filter value. For example, there is only one viral prep filter for promoter “Syn”, that, when selected, ...

  16. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...enough to ensure the virus can replicate and spread. Instead, viruses replicate by hijacking the host’s system...people in nature, extra safety measures, like replication incompetent viral particles, are required. We...

  17. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...post-transfection, excited at 505 nm (right). Scalebar represents 100 µm. Image from Abhi Aggarwal.   Brandán...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030. PubMed Central...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of interest...ccdB SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then... strains could possibly be used to propagate and prep your ccdB-containing empty backbones but should ...
Showing: 441 - 460 of 777 results