We narrowed to 798 results for: REP

...much more forgiving and can be mostly or entirely replaced with DNA bases while retaining activity in both...specifically recognizes its folds, especially the repeat:antirepeat duplex. Cas12 recognizes the 2’-OH of RNA ...

...booth about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...

...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against bacteriophages...for their ability to devise new strategies to reproduce within a host cell. A few virus particles have...

... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...most common expression system within the Addgene repository is mammalian, but the largest source of unique...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...error rates compared to other RTs. MarathonRT represents a major step forward in facilitating techniques.... Nature Methods. 2018. PMID: 29735997 bioRxiv preprint posted July 4, 2017 A new method to identify...

...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...biorender.com. Proper tissue processing and sample preparation can also help reduce the levels of endogenous...

... This allowed us to create pseudoviruses that represent lineages and variants that are actually transmitting... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...pseudovirus was similar to that of pseudoviruses representing a bat-derived coronavirus and SARS-CoV. These...

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

...flexible multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds...single guide RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...

...might like to store about FPs than is currently represented at fpvis (complete spectral information, accession...rapidly growing, with your help!), I believe this represents one of the largest available online collections...analysis tools, and a better way to store and represent within-study photobleaching comparisons.  We very...

...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

... organisms formally classified as fungi. They reproduce by either fission or budding, which is the primary...joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR). HR is...important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941 Bernauer...