We narrowed to 771 results for: SOM

...informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis by PCR obsolete...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing...

...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we...

...and so receives this valuable input from at least some employees. Watch out for "information allies" – ...in on percolating issues in the team.   Here are some other practices to encourage constructive input ...

...access (OA) movement might be going is to consider some of the places it’s been. So, as 2013 comes to a ...a close, let’s take a few minutes to reflect on some of the most noteworthy developments in open science...

...shared fascinatingly creepy science stories. Here are some of our favorite spooky science stories. Six spooky... blog Read about company culture at Addgene See some of our previous Halloween costume contests ...

... GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most ...problems in localization that have been encountered by some investigators when using forms of mEos." The ability...

...Protein purification specialists recommend doing some research, notably determining the subcellular localization...versatile and easy to use, and we hope that it will take some of the stress out of protein purification. For a...

...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases...

...that stack of papers on your desk, and to tackle some new experiments before the next R01 is due. But ...have added or removed samples. Once you've taken some time to get organized, stop back and let us know...

... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...proteins and complexes, without the artifacts sometimes associated with plasmid-based overexpression. ...

...Addgene’s homepage recently, you probably noticed some big changes to our site (if you haven’t, go check...kept pace with our growth over the last few years. Some of the navigation was buried on the page, and a ...

...AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV serotypes...usually be achieved by a directed brain injection, but some serotypes can cross the blood brain barrier after...

...out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...quarter we release a Newsletter where we spotlight some of the plasmids that have come through our doors...

...Scientists are excited by somewhat unusual things. For example, I am not that interested in meeting movie...panelists were interesting enough to make up for the somewhat naïve questions. During the panel, an artist was...

...built a Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational...cellular structures.The Davidson lab even included some of their own reference images that can be found ...

...our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months: A new lentiviral...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our ...

...manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can...

...dramatically reduced cleavage (Kim et al., 2020). For Cas9, some parts of the scaffold sequence could be substituted...? It’s not universal. In one base editing test, some hybrid guides increased off-target editing instead...

...eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during...

...hydrogen peroxide or ions like calcium and zinc. Some biosensors contain a single fluorescent protein ...information you are looking for. Below we have summarized some of the common fluorescent detection methods and ...