We narrowed to 749 results for: SOM
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TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...quarter we release a Newsletter where we spotlight some of the plasmids that have come through our doors...
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Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Scientists are excited by somewhat unusual things. For example, I am not that interested in meeting movie...panelists were interesting enough to make up for the somewhat naïve questions. During the panel, an artist was... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....built a Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational...cellular structures.The Davidson lab even included some of their own reference images that can be found ... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase...imaging shows only that the bacteria localize to somewhere in the mouse abdomen. arg1 signal, however, can... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through...experiments: Primer and restriction digest design Some regions of plasmids are difficult to sequence by... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though DA firing...concentration. To address this, researchers from some labs have developed fluorescent-protein based DA... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....hydrogen peroxide or ions like calcium and zinc. Some biosensors contain a single fluorescent protein ...information you are looking for. Below we have summarized some of the common fluorescent detection methods and ... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m.... processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they reduced by using superfolder...concerns are greater with SunTag. v1 SunTag exhibits some scaffold aggregation (v4 Suntag does not.) It’s... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months: A new lentiviral...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our ... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....manipulation tools Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses are able...avoids many, though not all, of these defenses. For some applications of genome engineering - such as certain... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating biological circuits...repository, and I was proud to be responsible for sharing some of our data at this meeting. We weren't quite sure... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m.... that aligns with your interests. We've outlined some important factors to consider and summarize helpful... “not be shackled by your own subject - maybe something used in astrophysics can be applied to fungal ... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....the lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is ...There are many excellent mentors out there. With some good pre-work you can find a lab that will deserve... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m.... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...we dug into the original literature, some of it decades old, to provide reliable feature annotations. ... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation of axons and... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....powerful tool to study proteins in vivo, it does have some limitations. GFP has long protein maturation times...amino acids, thus it is translated quickly by the ribosome. This feature provides HA frankenbody the ability... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....vectors (AAV) not all serotypes are equal; while some serotypes are typically high yielding, for others...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." ... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...-body mutants that respond to different pH’s and some that are fused to fluorescent proteins. She also...