We narrowed to 690 results for: eng

...experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to ...technologies currently available, you now face the challenge of trying to figure out what system might work...

...entire time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual...they are following along. This will not only re-engage you with those who sought out your poster, but ...

...post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby...blogger, Greg Dingle! Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on...

...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...

...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

...determinants of prime editing efficiency such as the length of the RTT overhang, the GC content of the primer... pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some...

...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...

...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

...viruses, with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

...transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of...2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis...

...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...scientists of all types to stay up to date and engaged with new advances in published science. They also...

...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

...negative sense RNA strand approximately 19 kb in length. It contains short promoter sequences at its 3’...replication and transcription, as it would for a full-length viral genome (Figure 2). While it is technically...

...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...

.... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...