We narrowed to 813 results for: org
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TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....Molecular Biology. Humana Press, pp 331–338 https://doi.org/10.1007/978-1-59745-181-9_23 Additional resources...about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids from Ginkgo Bioworks Find...
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Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m...., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...www.ncbi.nlm.nih.gov/books/NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...10):2073. DOI: 10.3390/v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m.... Boehm JS. Curr. Opin Syst Biol. 2017. http://dx.doi.org/10.1016/j.coisb.2017.02.002. We hope that by...About Cancer Immunotherapies Resources on Addgene.org Fine More Kits at Addgene Browse Plasmids from... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m.... would like to thank some of the people and organizations who helped us reach this milestone. Viral service...Research - A Primer for Beginners Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector ... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....basics. Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....are so ubiquitous in the lab that it is easy to forget that these enzymes naturally occur in bacteria ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....available along with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you... Kendall Morgan... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with... Kendall Morgan... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe. “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....Introduction to Antibodies Additional resources on addgene.org Antibody GuideRecombinant antibodies available...Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, N., Kim, Y., Lee, ...