We narrowed to 762 results for: SOM

...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Expanded...

...Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020). Some Acr proteins are specific to a particular Cas protein...

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...

...treatment should be apparent after three dsRNA feedings. Some of the phenotypes are not evident until after amputation...

... over the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-...

...trafficked and regulated. In this blog we will explore some of the most commonly used as well as newly developed...

...often performed via phage display, yeast display, ribosome display, or mammalian display (Tsuruta et al.,...

...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: New ...

...spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options for you to choose from...

...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...

...looser or different requirements. Luckily, there are some available.   Cas proteins that don’t utilize NGG...

...Workshop 2016 At past symposia, FoR worked to highlight some of the problems facing postdocs and graduate students...

...discovered, researchers wondered if it could overcome some of Cas9’s shortcomings. Cpf1’s preferred PAM is ...

...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

...comparing different batches of virus. In addition, some viral preps are very sensitive to freeze thaw cycles...

...substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden Gate...

...actually lower for xCas9 3.6/3.7 than for SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more ...

...blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other CRISPR...

...experiment?  Complexity of the gene of interest Some gene sequences are harder to amplify and quantify...

...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Axon...

...discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression ...

...make mistakes. What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck...

...truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, WLC, and...

...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

...used to perform genome-wide screens and highlight some of the reagents that have been made publicly available...

...of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...

...reaction is often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn...

...careful selection of FP fragments and, preferably, some information about the structures of the tested PIPs...

...activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries contain many sgRNAs per gene to...

... be incredibly valuable to scientists, there are some potential complications that must be considered ...