We narrowed to 507 results for: CIT

1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2]....

2. Genome-wide Screening Using CRISPR

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   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...

3. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

4. Uncovering Ancestral Bias in CRISPR Research

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   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ...their associated ancestries. While there were some exciting relationships identified, 48% were found to be...

5. DNA Purification Without a Kit

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   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

6. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying...

7. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...molecular biology of synaptic transmission and plasticity.     Javier García-Nafría is an investigator ...

8. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

10. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729...

11. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...your event in casual conversation to get people excited!    Laura Veckerelli is the Administrative and...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes...

13. CRISPR 101: Any Base Transversion Editors

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    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

14. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...concentrations (0.1-1% L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control...

16. Antibodies 101: Immunoprecipitation

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    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ... of interest. The wide availability and high specificity of anti-epitope antibodies make this approach...

17. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    ... B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized ...

18. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...Partnership Management, a very interesting and exciting part of my work. However, this is not the first...

19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers...

20. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...spread beyond its point of origin. One of the most exciting developments recently is a new blood test for ...