We narrowed to 504 results for: CIT

1. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

2. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ...their associated ancestries. While there were some exciting relationships identified, 48% were found to be...

3. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

4. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...molecular biology of synaptic transmission and plasticity.     Javier García-Nafría is an investigator ...

5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

7. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729...

8. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...your event in casual conversation to get people excited!    Laura Veckerelli is the Administrative and...

9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes...

10. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...Partnership Management, a very interesting and exciting part of my work. However, this is not the first...

11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers...

12. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...spread beyond its point of origin. One of the most exciting developments recently is a new blood test for ...

13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J mice is likely due to how the Deverman...

14. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...your off hours. DIY bio labs are cropping up in cities around the world, such as BosLab here in Boston...

15. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ... of interest. The wide availability and high specificity of anti-epitope antibodies make this approach...

16. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

17. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

18. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...concentrations (0.1-1% L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control...

19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    ... B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized ...

20. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...