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We narrowed to 867 results for: FER

Showing: 461 - 480 of 867 results

  1. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...York City, NY, USA 40.7128°N 74.0059°W – Deanna McLafferty I’m a New Yorker, so it’s not easy to convince...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...and fiction. Our health depends on it. Deanna McLafferty is an editorial assistant and activist. Washington...enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training...supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral ...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...inflammatory cytokines - interleukin (IL)-12 and interferon gamma (IFNγ) - and eliminate cancer cells. 2....physiological and morphological changes. Tumors differ in their genotypic and phenotypic identities based...by cancer. As mentioned in this article, cancers differ in practically every facet starting from manifestation...manifestation to their specific interactions with different cell types. A thorough understanding of the genotypic...interested in exploring new avenues in healthcare.   References 1. Vinay, Dass S., et al. "Immune evasion in ...

  3. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged ...packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this is just the beginning...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth...

  4. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... and Negative Selection for Plasmid Cloning by Jennifer Tsang There are many strategies for finding your...Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi...Precision and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique... Jennifer Tsang...

  5. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...validated gRNA sequences. Validated in this context refers to the fact that every gRNA listed in the table... generated from a plasmid. If you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins...we currently have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display...jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies...

  6. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...improve folding and disulfide bond formation. If one prefers expression in E. coli, the use of a thioredoxin...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    .... The following Friday, I received the official offer. I was so happy to hear back, I accepted that day... food service industry, which followed a very different schedule. Fortunately, I was provided with a detailed...gave me enough time to become familiar with the different types of issues that were important to this function...Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At this point...

  8. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ... value, clearly communicate exactly what a site offers to their users, and most importantly, allow those...get scientists excited about what Addgene has to offer, and to help scientists find what they need - whether...wanted to highlight our blog, daily updates, and offer better exploration throughout the site.  Blugene...2011, and while you may have seen our mascot at conferences, on our website, or in social media, we wanted...

  9. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...plasmids for expressing different types of rhodopsins and sensors as well as references and links to other ...using both its Addgene ID number as well as the reference for its original publication. To help facilitate... citation that you can put straight into your reference section. Why do this? Not only does citing plasmids...

  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...the future. Studies show that the majority of job offers arise as a result of existing professional (and...interest, even excitement, in a position. Everyone prefers to hire someone that will be excited to be there...resume should also be customized to highlight different skills and to get the important information in..., you will never get the help that can make a difference. Having your resume forwarded personally gives...

  11. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...eyes to the many different jobs associated with science, especially if the conference is a big multidisciplinary...curious and interdisciplinary.   3. “There are many different ways to succeed – as loner or gregarious;  dictator...leaving academia should present their research at conferences to obtain a “crash-course in all the real life...

  12. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...compare and analyze any differences between our sequence results and the reference sequences. Using Snapgene...features To align NGS sequencing results with reference sequences To design experiments for further plasmid...Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...

  13. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  14. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...

  15. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the spacer...scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins, either a crRNA...

  16. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...the discoveries yet to come.      References and resources References Arbabi-Ghahroudi M (2017) Camelid...become appealing candidates for the detection of different cancers (Jin, et al. 2023).  So where do all these...    Figure 2: Genetically modified mice offer an alternative method to producing single-domain...

  17. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ....  3. Do you prefer being a generalist? Working on a variety of projects and in different fields of research...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...

  18. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional approach of an autonomously...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these...YGOI on a western blot or under a microscope, we offer a collection of vectors carrying several fusion ..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...plasticware or think about conserving reagents and buffers.  However, I had very environmentally conscious...required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying..., I was bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took... small enough to minimize the required area and buffer volume.  So, I designed my own gel box (see illustration...

  20. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...paper is much more detailed. I prefer to do it on another day - preferably the following morning - as usually... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...
Showing: 461 - 480 of 867 results