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We narrowed to 777 results for: REP

Showing: 461 - 480 of 777 results

  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...all viral genes except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA)...transduced gene is episomal and does not divide and replicate in the same way the host DNA does. Because of ... since you don’t have to wait for the host to replicate the DNA before expression. Now that you’ve got...

  2. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...community. This is no small task, however, at a repository that consistently receives around 200 new DNA... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...

  3. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...perspective of both grassroots organisations that represent postdocs and institutes that employ them, the ...

  4. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won... days of file cabinets filled to the brim with reprints and papers with scribbled notations in the margins...

  5. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...couple of problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was... created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together very...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer...the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized ...

  7. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

  8. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   Figure 1:...genes E1 and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing...this “cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...the detection antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin...reached 4.0, you can resolve this issue when you repeat the experiment by diluting your standard curve,...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...

  11. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...hundred nl. Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing...ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral studies...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...and can be negative and/or even replicated studies to show reproducible results. This a great resource ...targeting vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...far more than it was worth to repair the controller and a new replacement was not possible, but one of ...  We’ve also learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...continued use inadvisable for experiments requiring reproducible results. Most cell culture media contain the...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...complicated if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency...very specific targeting, like homology directed repair and base editing, to more loci. Although these ...CRISPR Base Editing Learn about Homology Directed Repair Resources on Addgene.org Check out our CRISPR...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Fig. 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling...amplicons under 100 bp (Ma, 2015.) A schematic representation of the Taqman reaction can be seen to the left...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

  19. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...guides that target essential genes were less represented while bacteria containing guides targeting non-essential...
Showing: 461 - 480 of 777 results