We narrowed to 757 results for: nes
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TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....AAV9 is so strong that you can even see dendritic spines! Curious? Watch our AAV Data Hub Challenge recording..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work? We love negative...
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The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m.... 3,000 antibodies and associated hybridoma cell lines. The DSHB was created by the National Institute ...clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody against a ... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog PostPublishedFeb. 13, 2014, 1 p.m....rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can ...of published and commercially available empty backbones, visit Vector Database. Step 2: Insert name Include... -
What's New in CRISPR - May 2019
TypeBlog PostPublishedMay 28, 2019, 12:49 p.m....library (~60,000 gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs...APOBEC1 causes genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE ... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....biggest changes, there are plenty of less obvious ones to discover as you browse. You may find one of your...choices informed by Web Content Accessibility Guidelines (WCAG). But don’t take our word for it — check... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog PostPublishedFeb. 27, 2020, 2:15 p.m....online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at...to generate the legal agreement (MTA), which streamlines the process and saves you time. Anyone affiliated... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and...Most chromatogram viewing programs (even the free ones) allow you to edit the sequence. I hope these... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start something... publications like FierceBiotech to “learn the business” Attend events with local networking and trade... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....2006, we saw an increase in popularity of empty backbones for shRNA expression, such as pLKO.1 puro and ...shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....involved with COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…). How to...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....future. Over the years, we have benefited from the business rigor and efficiencies typically adopted by for-profit... Chonnettia Jones... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Public library. Being in the presence of scientific heroines is always inspiring. I was especially excited ...been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades in... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression...