We narrowed to 745 results for: nes
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TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ...
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Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....involved with COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…). How to...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....future. Over the years, we have benefited from the business rigor and efficiencies typically adopted by for-profit... Chonnettia Jones... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Public library. Being in the presence of scientific heroines is always inspiring. I was especially excited ...been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades in... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m.... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....Editing Consortium hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing...non-homologous end joining (Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: ... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR.... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....derived were tested for specificity in knock-out lines or animals. Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m.... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....patients suffering from Parkinson's, chronic pain, deafness, or other conditions. Addgene: You've contributed...require less power. Those are two of the biggest ones. And as a third example, it would be helpful to ...