We narrowed to 809 results for: org
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TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...
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Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....components of biological research that are often forgotten or ignored. If we wish to conduct research that...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....work or volunteer for a science communication organisation like Building with Biology. Local environmental...Science Communication Additional Resources on Addgene.org Find Job Openings at Addgene ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian, SP, et...Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....takeaway has been the impact I can have on an organization beyond my job description.” Our resident bagel...Addgene's science career guide Resources on Addgene.org Learn more about working at Addgene ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....choice for many behavioral studies, though other organisms such as rats or zebrafish are also used. With ...Beginner's Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....in research. Nature 518, 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe...Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on ... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m.... Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors Collection Split Luciferase...protein labeling. Nat Commun 8, 370 (2017). https://doi.org/10.1038/s41467-017-00494-8. PMID: 28851864.... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m..... Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007/s00018-019-03239-6 Additional resources...in our Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....antibody you used to assess the stripping, and don’t forget to re-block your membrane! Depending on the system...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene ...Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources Sequence Analysis of a... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV in Addgene's Viral Service... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....used to conversing with other scientists that we forget that some of the terminology we use routinely isn...applications for funding from charities, commercial organizations, or universities may have someone senior from... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m.... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently Asked Questions Pages... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...