We narrowed to 813 results for: org
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TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m.... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out...
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Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....components of biological research that are often forgotten or ignored. If we wish to conduct research that...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids ... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene ...Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources Sequence Analysis of a... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....antibody you used to assess the stripping, and don’t forget to re-block your membrane! Depending on the system...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV in Addgene's Viral Service... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m.... Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors Collection Split Luciferase... protein labeling. Nat Commun. 8, 370. https://doi.org/10.1038/s41467-017-00494-8. PMID: 28851864.... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m..... Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007/s00018-019-03239-6 Additional resources...in our Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....takeaway has been the impact I can have on an organization beyond my job description.” Our resident bagel...Addgene's science career guide Resources on Addgene.org Learn more about working at Addgene ... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....choice for many behavioral studies, though other organisms such as rats or zebrafish are also used. With ...Beginner's Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian, SP, et...Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer...