We narrowed to 762 results for: SOM

... be incredibly valuable to scientists, there are some potential complications that must be considered ...

...inducer virus. Additionally, this system is less cumbersome than brainbow, another method for multi-color...

...promoter. Although safer, this system may be more cumbersome to use and lead to lower viral titers due to ...

... that CRISPR, the bacterial immune system, may someday be used to target the hardest-to-kill bacteria....

... a different nuclease could potentially overcome some of Cas9’s shortcomings - namely its blunt double...

...high-throughput compatibility. However, there are some instances where we use long-read sequencing or Sanger...

...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...

...complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding their ...

...modifications Site-specific deletions and knockouts Some experiments may require the introduction of a mutation...

...References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding...

...research, the role of the MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches...

...  While finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver...

...mysteries of behavior. We’ve previously discussed some of the general considerations for performing behavioral...

...sequenced. Guide RNAs targeting several known and some unknown regulators of neuronal differentiation were...

...cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function in mammalian and plant...

...system SHERLOCKv2 for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less primer...

...suggest that the late replication that occurs for some types low copy plasmids during cell division simply...

...poorly at body temperature (37oC) and were too burdensome to express in situ. As a result, they had to ...

...of writing many of the specifics surrounding why some gRNA’s work better than others remain unknown, but...

...easy technique, but it is very important to follow some simple rules to avoid common pitfalls and to use...

...but its effectiveness varied depending on the chromosomal location, sequence context, and cell type. Multiple...

.... The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) increased...

...protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-...

...strategies to generate fusion proteins and highlight some aspects of their design.  Protein size and shape...

... CDC uses RT-qPCR tests to diagnose COVID-19 and some serological tests to determine past exposure. However...

...activates Spinach fluorescence. In the search for something more stable for quantitative imaging, Ming Hammond...

...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

...pSer-incorporated protein of interest as possible, some optimization will be necessary. To begin, one should...

...26721684. 4. Reardon S. Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi...

...tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis by ...