We narrowed to 504 results for: CIT

1. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...fusion with your favorite protein of interest is exciting and rewarding. But, what if an FP fusion with ...

3. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...significantly outperforms previous designs. B-gTEMP is excitable under blue light (470-495 nm) and is capable of...

4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ... fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights...

5. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than...

6. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ... similar to humans. For example, due to their simplicity, yeast have become an important experimental ...

7. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...resolvable, but will likely require a manager to explicitly assign the responsibility in question to a specific...

8. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ...therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to their lack...

9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...

10. Transferable Skills Guide: Time Management

    Type

    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...

11. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ... RNA endonucleases with customized sequence specificities." Nature communications 3 (2012): 1147. Cooke...

12. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...combination of host and target species, isotype specificity, and dye meets your needs and budget, or you ...

13. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

14. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...a cut at the target site. This is when it gets exciting – the cell sends out an “SOS” signal and cellular...

15. Deep Dive: Statistical Tests (Comparisons)

    Type

    Blog Post

    Published

    March 7, 2023, 2:15 p.m.

    ...long-awaited data – and it looks great! There are some exciting trends in your experimental groups, but how do...

16. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...graduate school via this report from the Scientific Citizenship Initiative.    Get practical experience in science...

17. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ... the audience. Ask yourself: What is the most exciting story I can tell with my data?  Can I make better...

18. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ... incubate your cells with virus at different multiplicity of infection (MOI=ratio of virus to cells). ...

19. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...-based. Their sequences can be optimized for specificity and sensitivity, for example by changing certain...

20. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...There are still many important questions in this exciting field of research. One of the most important: ...