We narrowed to 791 results for: REP

....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

...-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   Figure 1:...genes E1 and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing...this “cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale...

...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

...far more than it was worth to repair the controller and a new replacement was not possible, but one of ...  We’ve also learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never...

...Fig. 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling...amplicons under 100 bp (Ma, 2015.) A schematic representation of the Taqman reaction can be seen to the left...

...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...guides that target essential genes were less represented while bacteria containing guides targeting non-essential...

...into pieces small enough to be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride...the following changes to create CAPTURE 2.0:  Replace the biotinalatable tag on dCas9 with a BioTAP-tag...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original...

...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...continued use inadvisable for experiments requiring reproducible results. Most cell culture media contain the...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...

...complicated if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency...very specific targeting, like homology directed repair and base editing, to more loci. Although these ...CRISPR Base Editing Learn about Homology Directed Repair Resources on Addgene.org Check out our CRISPR...

...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...

... cell or its genetic material is damaged beyond repair, that cell will most often commit itself to the...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...commonly found across all TCGA tumor samples is represented, making the results of any such study as clinically...

...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...those of the guest blogger and do not necessarily represent the views of Addgene. Addgene performs Sanger ...greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide, although many...

...gene construct, you don’t have to worry about repeating elements anymore. The kit contains sequences for...about the advantages of talking to people and representing yourself as a real person, so that students ...doing. They look at structure, impediments, the representation of women on committees, and how the culture...

...the name suggests, you may also concentrate your prep to the desired concentration using these columns... this assay will also measure the purity of your prep. In this method, antibodies are separated by size...Contaminating bands lower the purity of your antibody prep. If you have multiple contaminating bands or a few...

...evil. However, it is easy to do sloppy science, misrepresent science, or misuse science, and that is what...of creating a technology that could eventually replace scientists themselves.  The sections on optogenetics...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...

...of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that... published on the Quilt Genomics Blog and is republished here with permission. Quilt is a collaborative...