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We narrowed to 777 results for: REP

Showing: 481 - 500 of 777 results

  1. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...into pieces small enough to be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride...the following changes to create CAPTURE 2.0:  Replace the biotinalatable tag on dCas9 with a BioTAP-tag...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Fig. 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling...amplicons under 100 bp (Ma, 2015.) A schematic representation of the Taqman reaction can be seen to the left...

  3. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... cell or its genetic material is damaged beyond repair, that cell will most often commit itself to the...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...commonly found across all TCGA tumor samples is represented, making the results of any such study as clinically...

  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...those of the guest blogger and do not necessarily represent the views of Addgene. Addgene performs Sanger ...greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide, although many...

  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...gene construct, you don’t have to worry about repeating elements anymore. The kit contains sequences for...about the advantages of talking to people and representing yourself as a real person, so that students ...doing. They look at structure, impediments, the representation of women on committees, and how the culture...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...

  7. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...evil. However, it is easy to do sloppy science, misrepresent science, or misuse science, and that is what...of creating a technology that could eventually replace scientists themselves.  The sections on optogenetics...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...the name suggests, you may also concentrate your prep to the desired concentration using these columns... this assay will also measure the purity of your prep. In this method, antibodies are separated by size...Contaminating bands lower the purity of your antibody prep. If you have multiple contaminating bands or a few...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...get Addgene from a concept to an up and running repository. As they built the foundation for what Addgene... part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene...things!!”  Okay - honestly, maybe not everyone replies with quite so much animation, but the gist is a...

  11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...in some strains of mice, these results are not reproduced when the vector is used in different mouse strains...with mouse-strain dependent performance. This surreptitious finding illustrates how the method used to select... mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene. References Holmes,...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...transition from the bench can be jarring for the unprepared, and unfortunately, there aren’t a lot of resources...involves a few key features that can be difficult to replicate in other work settings: The sense of being the...question as well then as I would now. I would have to repress laughter if I was asked that question today, because...

  13. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...interpret the results, and develop a successful, reproducible assay. That information can make you a much ...email. Notes from real-life hiring managers In preparation for updating this post, I took an informal poll...applicants. In short, while a cover letter can’t replace skills and experience, it can help the hiring manager...

  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...from broadest to most specific marker, and are represented as a series of dot plots in the same order. However...2018, uses quadrants to gate and has numbers representing the percentage of cells in each gate. Used under...

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...option.  Target protein/antibody interactions To prepare for an IP, samples are typically lysed in a non-denaturing...protein/capture antibody intact. The wash step is repeated several times to ensure non-specific proteins ... (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...steps within its own container because of the reproducibility containers provide and for convenient compatibility...processes correctly. After this validation check, we repeated our strategy and copied a few lines of our existing...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide... all, of the FP fusions. Our moxFPs currently represent one of the most inert solutions for imaging in...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

  18. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...activation of the circuit promotes subsequent, repeated activation), this is an approximation of reward...instills a sense of “reward”), then the animal will repeatedly nose-poke at the active hole [1, 2].    Place...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

  20. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...
Showing: 481 - 500 of 777 results