We narrowed to 798 results for: REP

...couple of problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was... created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together very...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...

...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer...the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized ...

...hundred nl. Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing...ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral studies...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

...and can be negative and/or even replicated studies to show reproducible results. This a great resource ...targeting vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen...

...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

...the detection antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin...reached 4.0, you can resolve this issue when you repeat the experiment by diluting your standard curve,...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...

....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

...-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   Figure 1:...genes E1 and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing...this “cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale...

...far more than it was worth to repair the controller and a new replacement was not possible, but one of ...  We’ve also learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never...

...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...guides that target essential genes were less represented while bacteria containing guides targeting non-essential...

...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...

...Fig. 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling...amplicons under 100 bp (Ma, 2015.) A schematic representation of the Taqman reaction can be seen to the left...

...into pieces small enough to be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride...the following changes to create CAPTURE 2.0:  Replace the biotinalatable tag on dCas9 with a BioTAP-tag...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original...

...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...continued use inadvisable for experiments requiring reproducible results. Most cell culture media contain the...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...

...complicated if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency...very specific targeting, like homology directed repair and base editing, to more loci. Although these ...CRISPR Base Editing Learn about Homology Directed Repair Resources on Addgene.org Check out our CRISPR...

...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

... cell or its genetic material is damaged beyond repair, that cell will most often commit itself to the...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...commonly found across all TCGA tumor samples is represented, making the results of any such study as clinically...

...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...those of the guest blogger and do not necessarily represent the views of Addgene. Addgene performs Sanger ...greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide, although many...