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  1. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... as an ideal target. It is an easily accessible organ that can be monitored non-invasively over time. ...for gene expression in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's..., 2014, Pages 438-449, ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz...

  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org/10.1101/2022.11.18.517155   Resources on ancestral...diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586-022-04601-8 Lewis, Anna CF, Santiago...

  7. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview... Kendall Morgan...

  10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search...

  11. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...Management Advice from Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...Entrepeneur Get Tips on Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources ... With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...Review. Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity...Review. Retrieved January 14, 2022, from https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276.   Optogenetic...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., ...Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089/hgtb.2017.198  ...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ...

  18. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below... Series Science Career Options Resources on Addgene.org Learn more About Careers at Addgene ...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR...
Showing: 481 - 500 of 796 results