Skip to main content
Addgene
Showing: 251 - 260 of 602 results
  1. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...
  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...
  3. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...
  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...
  5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of... for an “escape” virus to propagate and infect adjacent cells. It will be important to determine if CRISPR...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...
  6. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... characterised antibodies against all human extracellular proteins with community-based validation. They...experiments on similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000...their experimental designs. There are many more facets to reproducibility issues in the biological sciences...
  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more...
  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...you want to know if they co-localize at the same place in the cell? Or you know that the presence of several...your microscope. Abcam's sample protocol is a good place to get started.  One important consideration when...the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split Channels...
  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...GAG), and are (typically) tethered to the cell surface by a GPI-anchor. Image courtesy of IPI.   ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...
Showing: 251 - 260 of 602 results