We narrowed to 791 results for: REP

...get Addgene from a concept to an up and running repository. As they built the foundation for what Addgene... part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene...things!!”  Okay - honestly, maybe not everyone replies with quite so much animation, but the gist is a...

...become a key focus in recent years. TnpB nucleases represent an exciting class of compact genome editors, typically...TEEP — a tool based on animal data. Step 2: Prepare Oligos Oligo 1: Add 'tcaa' to the 5′ end of your... cells. Conclusion & Future Directions TnpB represents a new generation of compact, efficient, and versatile...

...fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done all the learning...number of assemblies, you will also likely need to miniprep a lot of plasmids from your bacterial stocks beforehand...protocol shared by Dr. Rob Hurt for high-throughput minipreps in 96-well plates. Plate wisely You will likely...

...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...steps within its own container because of the reproducibility containers provide and for convenient compatibility...processes correctly. After this validation check, we repeated our strategy and copied a few lines of our existing...

...option.  Target protein/antibody interactions To prepare for an IP, samples are typically lysed in a non-denaturing...protein/capture antibody intact. The wash step is repeated several times to ensure non-specific proteins ... (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol...

...in some strains of mice, these results are not reproduced when the vector is used in different mouse strains...with mouse-strain dependent performance. This surreptitious finding illustrates how the method used to select... mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene. References Holmes,...

...transition from the bench can be jarring for the unprepared, and unfortunately, there aren’t a lot of resources...involves a few key features that can be difficult to replicate in other work settings: The sense of being the...question as well then as I would now. I would have to repress laughter if I was asked that question today, because...

...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...from broadest to most specific marker, and are represented as a series of dot plots in the same order. However...2018, uses quadrants to gate and has numbers representing the percentage of cells in each gate. Used under...

...interpret the results, and develop a successful, reproducible assay. That information can make you a much ...email. Notes from real-life hiring managers In preparation for updating this post, I took an informal poll...applicants. In short, while a cover letter can’t replace skills and experience, it can help the hiring manager...

...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide... all, of the FP fusions. Our moxFPs currently represent one of the most inert solutions for imaging in...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

...activation of the circuit promotes subsequent, repeated activation), this is an approximation of reward...instills a sense of “reward”), then the animal will repeatedly nose-poke at the active hole [1, 2].    Place...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

...your insert of interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of...(see Figure 2).  Figure 2: Schematic representation of integration process and episomal forms of...dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However, IDLV genomes...

...sequence. The TIDE software will generate a graph representing all insertions and deletions identified within...Cas9 combo and are hoping for homology-directed repair events, or maybe you’re performing a single base...TIDER can screen for specific homology-directed repair events and small edits (Brinkman, et al., NAR)....

...depend on the target and sample type. Sample Preparation This step can involve multiple smaller steps...Preservation and Imaging Similar to Sample Preparation, this step can involve multiple smaller steps...especially if you work in an organism that is underrepresented in the biomedical research community. You ...

...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

...activation loop do not fully activate MEK1. In fact, replacing just one of the MEK1 phosphomimetic residues in...to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This modification allowed the...expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using...

...ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in vivo, which...large, has a high GC content, contains a lot of repeats--any of which could make the PCR step difficult...

...is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep. In...

...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...spurious yet intriguing findings that fail to replicate. Plan experiments logically and strategically ...perform, and interpret experiments. You'll be better prepared for your future intended career and you'll become...