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We narrowed to 777 results for: REP

Showing: 501 - 520 of 777 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...your insert of interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of...(see Figure 2).  Figure 2: Schematic representation of integration process and episomal forms of...dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However, IDLV genomes...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

  3. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sequence. The TIDE software will generate a graph representing all insertions and deletions identified within...Cas9 combo and are hoping for homology-directed repair events, or maybe you’re performing a single base...TIDER can screen for specific homology-directed repair events and small edits (Brinkman, et al., NAR)....

  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...activation loop do not fully activate MEK1. In fact, replacing just one of the MEK1 phosphomimetic residues in...to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This modification allowed the...expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep. In...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...responsible for guiding new deposits into the repository, performing quality control, and providing technical...listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized I could and should be...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...spurious yet intriguing findings that fail to replicate. Plan experiments logically and strategically ...perform, and interpret experiments. You'll be better prepared for your future intended career and you'll become...

  9. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...depends on a variety of factors including sample preparation, staining procedures, and controls. To prevent...multicolor flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation...

  11. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to reach out ...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

  12. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over...applications (such as visualize, screen, activate, and repress) on Thursday. We’d also like to see what you think...

  13. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...

  14. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

  15. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of our...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... a trick to get the highest yield out of your minipreps? If so, we’d love to have you write for us! Click...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

  17. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

  19. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...longevity. 10:56 - 12:00 : How Alex's work in DNA repair informed his decision to work with CRISPR during...Koeris Explains His Path from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career...

  20. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...
Showing: 501 - 520 of 777 results