We narrowed to 580 results for: RON

1. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...quality. But even journal-based peer review can be prone to biases or errors, with a lack of transparency...

2. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...10kD synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead ...

3. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...

4. Click Biology: How to Click and Play to Explore Biology

   Type

   Blog Post

   Published

   Oct. 21, 2025, 1:15 p.m.

   ...Let Dye: Self-Labeling Protein Tags Plasmids 101: Degron Tags Additional Resources on addgene.org New ...

5. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ... resources, and, on the rare occasion things go wrong, replace them for free. Plasmids ship out 2-3 days...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...many FRET-opsin GEVIs. With an increase in the neuron’s membrane potential, Ace absorbance increases and...

7. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...repetitive regions (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors...

8. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...multimers using class-IIS restriction enzymes. Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC. Genet Anal.1996...

9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically challenging...

10. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...control mice will have been raised in the same environment and will harbor similar epigenetic modifications...

11. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...transducing a cell line of interest a lot can go wrong. We hope that we have provided you with a few ideas...

12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...combinations and pick the conditions that give the strongest biotinylation signal, as measured via Western ...

13. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...activation can occur through fusing dCas9 with VP64, a strong transcriptional activation domain. Guided by dCas9...

14. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    .... Either way, just let the interviewers know up front how you plan to take questions.  Relax!  You wouldn...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...some older split-GFP constructs or certain cell environments). Also consider that a protein fusion may disrupt...

16. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...organisation like Building with Biology. Local environmental groups, charities and NGOs are often looking...

17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids...

18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... For plasmids with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral...

19. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ...manipulation to produce and edit plasmids. These are prone to error — both human and biological. Even the most...

20. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...