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We narrowed to 749 results for: SOM

Showing: 501 - 520 of 749 results

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...protein expression control tools as well as explore some new innovations. Whether you want to turn things...more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out our Cre ...

  3. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

  4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...

  7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...methods aren’t stealthy enough to gain access to some cells and tissues, such as human induced pluripotent...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

  8. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...experiments.  In this blog post let's take a look at some of the other open resources discussed at the meeting... (Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared...

  9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... from Maya Kostman for the IGI.     Here are some highlights of the plasmids shared over the last ... plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository for...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...visual person. Why don’t you record a podcast or put some effort into making a video? Use your unique talent...annual meetings. 8) Training – Get a formal education Some people get into science communication by taking ...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...to select large sets (with overhangs that violate some of the traditional design rules) for successful ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...ability to be metabolized into clozapine (CLZ). While some scientists argue that these issues can be mitigated...These synthetic agonists can penetrate the brain and some, such as varenicline, are already approved for clinical...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... of reasons for this but the primary one is that some behavioral experiments require stimulation only ... in this primer on optogenetics we’ll delve into some of the important considerations when performing ...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... need to collect will depend on the serotype, as some rAAV serotypes are abundantly released into the ...10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer...

  16. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your...specific value between the two channels. It is something your computer will do for you to better analyze...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you...that cross-contamination can be identified. Even if some mammalian cells lines have similar morphologies,...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...reached your lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    .... Additionally, these transposon also exhibit something called target immunity, whereby multiple proximal... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given single...Exchange) system (Abudayyeh et al., 2019). To avoid some of the inherent drawbacks for RNA editing of natural...
Showing: 501 - 520 of 749 results