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TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m...., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...
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Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org/10.1101/2022.11.18.517155 Resources on ancestral...diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586-022-04601-8 Lewis, Anna CF, Santiago... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview... Kendall Morgan... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276. Optogenetic...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., ...Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089/hgtb.2017.198 ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources ... With Cas13 CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....Review. Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity...Review. Retrieved January 14, 2022, from https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...Management Advice from Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....gene gets its name based on what happens to an organism when a gene/protein is depleted in a knockout ...Read our Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research Deposit plasmids... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...Entrepeneur Get Tips on Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below... Series Science Career Options Resources on Addgene.org Learn more About Careers at Addgene ... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...Check out Plasmid Cloning by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Genome-Wide Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m.... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...19408315; PMCID: PMC2735187. More resources on Addgene.org Addgene's Plasmids 101 eBookAddgene's Molecular...