...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...we used to drive recombinase expression may be replaced by sensors that correspond to the desired inputs...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple...

...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with a human gene. Advantages...comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater...

...restriction enzymes that allow your insert to be placed into the backbone in the proper orientation. For... restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

...of the world, professional internationalization faces several obstacles; for instance there are few resources...career are common problems that many others have faced yet, still, this challenge did not hinder them from...evaluation of the pilot program IMP 2014/2015, took place on July 27th, 2015 at a dedicated international ...

...packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming a vector that ... Alternatively, your gene and the marker can be placed under the control of two different promoters. Both... Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer vector...

...Overview of using Lambda Red recombineering system to replace a gene of interest with an antibiotic resistance...as non-selectable DNA fragments, such as gene replacements and tags.  The typical frequency of recombinants....e. make changes to the third base pair of the adjacent 4-5 codons that alter the nucleotide sequence ...

...teamwork was what drew me to the school in the first place. The famous discoveries and experiments I’d been...at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that nickname...sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages of team-based...

...that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect on your personal... next four years. But college, and science, are places for learning and growth! As you learn more about....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

...government, evidence to inform policies, a protected place to work, funding for science, and numerous other...every political persuasion) must be humble in the face of evidence. Stephanie Hays is moving from engineering...Harvard University to studying an evolutionary arms race at University of California, Berkeley. She is a ...

...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use..., 2019). LY6A is functionally expressed on the surface of brain endothelial cells in C57BL/6J mice, but...