We narrowed to 554 results for: ESP
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TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....stoichiometry of donor:acceptor is difficult to address especially when you investigate unknown molecular complexes...
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Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....activate the Rho GTPases Cdc42, Rac1, and RhoA, respectively) using the iLID system (Mahlandt et al., 2023... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....models of human AML accurately predict chemotherapy response." Genes & development 23.7 (2009): 877-889. PubMed... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....used to do mammalian cell culture, I would often respond to friends asking me to dinner saying something... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will fluoresce either red or orange until... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....clearly not equitable to that of other trainees despite the fact that the underrepresented individual is... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....possible are present in most commercial strains, especially mutations that make major improvements such as... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....correlate with catalytic activity at those sites, especially when DNA mismatches are involved. Two other approaches... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m...., Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As the effects... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....for integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s ... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m.... very strong beliefs in traditional medicine, especially when I knew that there was a cheap and easy treatment... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more efficiently... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant...