We narrowed to 557 results for: ESP

1. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...titer virus is used, it is not always feasible, especially when the lentiviral stock needs to be titrated...

2. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References...

3. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ...stoichiometry of donor:acceptor is difficult to address especially when you investigate unknown molecular complexes...

4. The Advances Behind The World's First Personalized CRISPR Treatment

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   Blog Post

   Published

   Sept. 16, 2025, 1:15 p.m.

   ...produced synonymous changes, meaning the new codon corresponded to the same amino acid. Thus, bystander editing...

5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...

6. Five Popular Model Organisms

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   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ...models of human AML accurately predict chemotherapy response." Genes & development 23.7 (2009): 877-889. PubMed...

7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will fluoresce either red or orange until...

8. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   Published

   Oct. 5, 2021, 1:15 p.m.

   ...miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These...

9. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...activate the Rho GTPases Cdc42, Rac1, and RhoA, respectively) using the iLID system (Mahlandt et al., 2023...

10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our...

11. Transferable Skills Guide: Time Management

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    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ...used to do mammalian cell culture, I would often respond to friends asking me to dinner saying something...

12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post

    Published

    May 5, 2020, 1:15 p.m.

    ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...

13. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially...

14. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

15. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...possible are present in most commercial strains, especially mutations that make major improvements such as...

16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As the effects...

17. CRISPR 101: Off-Target Effects

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    Blog Post

    Published

    Feb. 8, 2024, 2:15 p.m.

    ...correlate with catalytic activity at those sites, especially when DNA mismatches are involved. Two other approaches...

18. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ..., Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator...

19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

20. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...for integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s ...