We narrowed to 566 results for: FIE

1. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...Christine Nusslein-Volhard and Eric Wiechaus first identified the hedgehog gene in Drosophila. They observed...

2. Transferable Skills Guide: Creativity

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   Blog Post

   Published

   Jan. 18, 2022, 2:15 p.m.

   ...In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand in the way of creative...

3. Antibodies 101: Immunoprecipitation

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   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ...translatable to the endogenous system and will need to be verified using alternative methods.  Finally, IP can also...

4. Viral Vectors 101: Pseudotyping

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   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...F-L (2002) Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability...

5. Overcoming the AAV Size Limitation for CRISPR Delivery

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   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In general split AAVs...

6. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing via CRISPR...

7. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ... (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 for example...

8. 5 Reasons to Use Reddit for Science Communication

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   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...with people who work in many different scientific fields. They also host AMA forums where users can ask ...

9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...encode N-glycosylation consensus sequences are modified with the addition of N-glycans [9]. Both of these...

10. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ... as inclusion bodies, and can be recovered and purified using a special protocol. Alternatively, reducing...

11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...regards to integration, and how researchers have modified them to prevent this integration.  Lentiviruses...

12. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...done on live cells. For cellular proliferation, esterified dyes that can enter cells freely are typically...

13. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...with homologous DNA to the target sequence to be modified. In general, the insertion sites of the modification...

14. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...vectors (here and here).  Figure 3: JEDI-2P was identified from a high-throughput screen, selected for increased...

15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ... we tested a strategy that was discussed in the field at the time but had never been systematically analyzed...

16. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link between...

17. Five Popular Model Organisms

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    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling." Proceedings of ...

18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ... The localization of the fusion protein can be verified and it can be compared to what is expected based...

19. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...compromise could leave everyone feeling vaguely unsatisfied. In arbitration, there is more of a winner and...

20. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...finally found my footing with the company. I’d identified the ways to get the most out the resources we...