We narrowed to 610 results for: REV

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...

2. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly...

3. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...proteins available. Fortunately, several excellent reviews have collated data from various papers to make ...

4. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR...

5. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ...benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA...

8. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ...

9. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...

10. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...(NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful...

11. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...adult-born granule cells over time that were not previously possible.   Bensussen S, et al., iScience 2020...

12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

13. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... libraries were originally described in a peer-reviewed scientific publication. We love science, but we...

14. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael...

15. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional titer...

16. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ... initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation...

17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello presented ...

18. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...need to be polished to significant smoothness to prevent drastic loss of efficiency between patch cable ...

19. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...some instances I felt like I was going back to previous formats but I learnt to overcome the personal ...

20. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...