We narrowed to 610 results for: REV

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ...

2. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...proteins available. Fortunately, several excellent reviews have collated data from various papers to make ...

3. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR...

4. Plasmids 101: Modular Cloning

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   Blog Post

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   March 12, 2024, 1:15 p.m.

   ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ...

5. Progress Towards a PAM-Free CRISPR

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   Blog Post

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   March 7, 2024, 2:39 p.m.

   ...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...

6. Hot Plasmids February 2024

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   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...(NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful...

7. Viral Vectors 101: Calcium Sensors

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   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous...

8. Viral Vectors 101: Chemogenetics

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   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   .../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ...

9. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

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   Oct. 8, 2024, 1:15 p.m.

   ...Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...

10. Antibodies 101: Choosing the Right Isotype

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    Blog Post

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    April 6, 2023, 3 p.m.

    ...choice affect my experiment? In this post we will review some of the main factors that affect isotype choice...

11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...issues.” We then ask the depositing laboratory to review the discrepancies. If the depositing laboratory...

12. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair...

13. Tips for Technical Support Calls

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    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... libraries were originally described in a peer-reviewed scientific publication. We love science, but we...

14. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael...

15. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional titer...

16. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ... initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation...

17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello presented ...

18. The Materials Science of Optogenetics Experiments

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    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...need to be polished to significant smoothness to prevent drastic loss of efficiency between patch cable ...

19. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...some instances I felt like I was going back to previous formats but I learnt to overcome the personal ...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...low increase in NGA editing efficiency fits the previous observation that NGA is a secondary PAM for SpCas9...