We narrowed to 610 results for: REV
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TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...
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Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....proteins available. Fortunately, several excellent reviews have collated data from various papers to make ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....(NHP) brains. Systemic gene delivery capsids previously identified in rodents have not been widely successful... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....adult-born granule cells over time that were not previously possible. Bensussen S, et al., iScience 2020... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m.... libraries were originally described in a peer-reviewed scientific publication. We love science, but we... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....concentrating a viral stock. Different titering methods can reveal differences in physical versus functional titer... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m.... initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello presented ... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....need to be polished to significant smoothness to prevent drastic loss of efficiency between patch cable ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....some instances I felt like I was going back to previous formats but I learnt to overcome the personal ... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...