Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 817 results for: nin

Showing: 521 - 540 of 817 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...world manifesting as a full-fledged pandemic. Containing the spread of this virus has become an utmost...earlier epicenters of the outbreak ultimately flattening the curve, it has become evident that widespread...beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... E., & Gomes, A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western...more accurate as a loading control. Thanks for joining us in this exploration of western blot normalization... Gomes, A. V. (2013). Stain-Free total protein staining is a superior loading control to β-actin for Western...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...(1:24 - 29:31), there are a variety of ways of defining scientific reproducibility. For a study to be ...even simple procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned...the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit more about...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies...absorption by tissues. To avoid absorbance of heme-containing proteins in whole animals, light at or above ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic...Epidermal growth factor receptor (EGFR)3 AAV8 Laminin receptor (LR)3 AAV receptor (AAVR)2 Carboxypeptidase...Terminal N-linked galactose3 AAV receptor (AAVR)2 Laminin receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...2], [3]. Simply put, the piggyBac™ transposon containing a selection marker is included in a standard ...template to facilitate the selection of cells containing your desired edit. The selection is marker is...transposase enzyme [4]. By using a donor plasmid containing a negative selection marker such as thymidine...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...degraded. Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment...matter of preference.   Which size kit to use?  Obtaining higher quantities of DNA generally means starting...

  9. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me: RNA editing with...

  10. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...the second year in a row, agar art is one of the winning decorations for Deck The Lab. These festive, living...Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565 — Jess ...

  11. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ... to basic lab etiquette that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick...and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them...

  12. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... us to ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international...allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus...

  13. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...Hassabis and John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding...least that we’ve seen so far!). They started by designing a novel topology, then iteratively generated 3D...

  14. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...

  15. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for Synthetic...more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial genome...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...your blog on a variety of social media outlets meaning you’ll have the opportunity to interact with and...different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ... costumes). All of this costume planning is for a good cause… Winning! The team with the best costume ...

  18. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...druryhannah) The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition..., 2020   Deck the Lab: Home Edition Winners Winning first place in the home edition, the MicrobiGals...
Showing: 521 - 540 of 817 results