We narrowed to 695 results for: prot

... start shipping. They created the best safety protocols they could with limited guidance. We were also...applied for the government sponsored Paycheck Protection Program loan and received enough funds to make...

...recombinant antibodies, educational materials, protocols, and shared data, on our values of transparency... antibody collection.  Addgene is sharing our protocols for recombinant antibody production as well as...

...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...

...transcriptional regulator Their system is based on the AraC protein that controls transcription from the PBAD promoter...blocked by the mask will not produce the fluorescent protein while areas that are exposed will fluoresce. With...

...produce a subset of this collection as ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure... and we plan to introduce affinity reagents in protein format within the next year. NIH grants and Addgene...

... your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to highlight...

...in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...Xiuyuan Ou, et al. that characterized the spike (S) protein of SARS-CoV-2 (Ou et al., 2020). The authors requested...Wang J, Qian Z (2020) Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune ...

...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM...student who has spent years studying how that one protein interacts with that one promoter can tell you, ...

...verification to find additional tips and detailed protocols on topics such as how to set up your digests and...Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

...170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated...Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization...

...only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features, such as...between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align...

...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated...

...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

...sensitivity are key considerations in choosing a Cas protein for your experiment. References Hu, Johnny H....101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources on Addgene.org...

...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

...strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will require...A: At CLOVER, we have traditionally used qPCR (protocol link). We have found this method to be reliable...

...to be due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson...virus-specific elements such as the LTRs or structural proteins are not interchangeable between the two systems...

... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...

...most recombineering strategies, only 3-4 phage proteins are needed and these can be provided via a separate...transformed into bacteria expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous...