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TypeBlog PostPublishedJuly 6, 2023, 1 p.m....monitor with biosensors? You can keep tabs on my personal favorite (the caffeine of the cell!)—ATP. There...
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GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m.... Rachel Leeson... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....Haery in 2017 and significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....electroporation of a human, for example, isn’t a reasonable option. Since viruses used in labs are derived... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog ... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....mediated by some kind of communication to another person. In an interview setting, this means persuading... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....in the graphic below - including some simple comparisons. The easy multiplexing afforded by PTGs will... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), and try to find the... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable Fluorescent Proteins... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and transcriptome... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....scientific communication needs. The most often-cited reasons researchers might not post a preprint are reluctance... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... Rachel Leeson... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m.... the brain. References Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....is a genome engineering powerhouse, and for good reason. It is guided by an RNA molecule, the gRNA, to ... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....purification without a kit that outlined several reasons why doing something without a kit has advantages... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....will still recognize your protein. For the same reason, they’re also useful in experiments where the availability... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....loci Building on the work of Chen et al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct...