We narrowed to 554 results for: ESP

1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...expression of the int and xis genes which are responsible for excision of mini-λ from the host’s chromosome...

2. Live and Let Dye: Self-Labeling Protein Tags

   Type

   Blog Post

   Published

   April 16, 2024, 1:15 p.m.

   ... HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more efficiently...

3. Deep Dive: Statistical Tests (Comparisons)

   Type

   Blog Post

   Published

   March 7, 2023, 2:15 p.m.

   ...family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant...

4. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ... precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA) is conceptually similar...

5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ... problem. It is estimated that mycoplasma is responsible for up to 60% of the cell culture contamination...

6. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   Published

   Feb. 25, 2020, 2:15 p.m.

   ...present-day frogs via genetic engineering. The corresponding movie came out in 1993 and sequels of Jurassic...

7. Plasmids 101: Modular Cloning Applications and Kits

   Type

   Blog Post

   Published

   May 14, 2024, 1:15 p.m.

   ...attention to protein modifications which can be especially difficult in alternative expression systems such...

8. A Primer on Optogenetics: Introduction and Opsin Delivery

   Type

   Blog Post

   Published

   Sept. 10, 2015, 2:30 p.m.

   ...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably...

9. CRISPR 101: Cytosine Transversion Editors

   Type

   Blog Post

   Published

   March 25, 2025, 1:15 p.m.

   ...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...

10. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...workflow.) Our spreadsheet contains columns that correspond to the plasmid data we need; you can copy and...

11. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells. Scientists everywhere wistfully...

12. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...function or cause cellular growth delays. With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing...

13. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...single synaptic step, thus preventing further widespread expansion of RABV. This improvement resulted ...

14. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...for spatial proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation...