...Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...

...a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church ...gene drives focusing on safety and the ability to generate mutant microbial strains faster than ever before... Advisory Board with a particular interest in generating Cas9 based tools to enable facile control of ...

...service to further contribute to our mission to “accelerate research and discovery.” Here are the details...in the research on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...

...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene headquarters every day. That would be an average of 673 plasmids each day. This requires tremendous...

...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...

...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the...Pay by Purchase Order.” Enter the PO number you generated through your organization in this field. Please...purchasing department requires a quote in order to generate a PO, learn more about how to create a quote. ...

...Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid... not just sharing research materials — we're accelerating the future of science. Want to learn more about...

...and in my personal experience, is a quick way to generate many mutated plasmid constructs. The second post...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

...communicating science to the general public and training the next generation of science communicators. In... is a source of scientific information for the general public as well as a platform for scientists to ...

... and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic lethal, hindering ... titer lentivirus preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!...., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains...

...languished in the pages of thick textbooks are now liberated to adorn the walls of universities and the front...Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer...aspect alone. They can study molecules, analyze terabytes of information on black screens, but not to know...

...organizations to share materials more broadly to accelerate COVID-19 research: Sinai Health System                                     ... available to industry? Over the past 8 years, several organizations have made their plasmids available...Shop’ for Fluorescent Proteins, and plasmids from several labs at Florida State University became openly ...

... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international...

...seqWell and going into detail about how we use next generation sequencing results to perform quality control... We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an...is always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the ...

...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...more mysterious. Thus, scientists have developed several ways to sense dopamine. Methods for measuring Dopamine...Pavlovian conditioning Find the GRABDA sensor here! Overall these sensors are powerful new developments in ...

...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...

...developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (Fig. 1):  ...Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system, HDVR is placed upstream of the...organisms including mouse and fish, SPARC is likely generalizable to other systems as well. References: del...

...resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to increase... Users can cross-interrogate screens from the literature for a drug, disease, or condition of interest...

...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...and the insert size should be a max of 500 bp (preferably). I compiled a list of three options from the...Through Addgene, I learned the basics of lab safety, general work skills — such as using Google workspace — ...

...needs to integrate within an exon of a gene to generate a transcriptional fusion. This may disrupt the...reading frame, in fact, they can be activated a considerable distance away from a gene which can make identification...there are many ways to insert your construct to generate a collection of monitored genes and mutants - ...