We narrowed to 566 results for: FIE

1. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...finally found my footing with the company. I’d identified the ways to get the most out the resources we...

2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...

3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...worldwide. As we’ve previously seen in the CRISPR field, the lessons learned in pursuing one technological...

4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...Gibson assembly. In this case the vector was PCR amplified to contain regions of homology with the bacterial...

5. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...Tabebordbar et al. show that muscle stem cells are modified by this approach, increasing the possibility that...

6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...fluorescent proteins, with recombination events specified by three loxP variants, but the principles remain...

7. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 2:15 p.m.

   ...there are up to thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition...

9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...

10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ... that it requires a high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah...

11. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ..., and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used under...

12. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...modification. Whole-genome sequencing of cells modified by CRISPR indicates that the consequences of off-target...

13. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with...

14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ... annealed to target DNA (genomic DNA or a PCR amplified fragment) and treated with a high fidelity thermostable...

15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...genome browser and Primer3 to design primers that amplified a 500-600 bp product. The primers I designed (...

16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...within the brain, researchers inject genetically modified viruses encoding microbial rhodopsins into specific...

17. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ... technologies have brought a renaissance in the field of health sciences, energy, and agriculture, its...

18. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...Next, you learned about how fluorophores can be classified into different levels of brightness and their...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ... and then passing the cells through an electric field to deflect them into appropriate containers. The...

20. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...March, three climate scientists and an economist testified before the House Science Committee about climate...