We narrowed to 610 results for: REV

1. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   Published

   April 18, 2024, 1:15 p.m.

   ...authorial contributions to, and support for, this and previous Addgene CRISPR publications.  References and ...

2. Of Myc and Men

   Type

   Blog Post

   Published

   Jan. 19, 2023, 2:15 p.m.

   ...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

3. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...; at least you know you have an answer to that previous nosy question! The Application Process Broadly...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...low increase in NGA editing efficiency fits the previous observation that NGA is a secondary PAM for SpCas9...

5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...Drosophila geneticists use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially...

6. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...new knockout animal models in species that were previously difficult to engineer, like rats. If you have...

7. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...entire conference, in general). After the conference Review your favorite conference moments on social media...

8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...(ICEs): what they do and how they work." Annual review of genetics 49 (2015): 577-601. PubMed PMID: 26473380...

9. Academic vs. Industry Postdocs

   Type

   Blog Post

   Published

   Nov. 13, 2018, 1:22 p.m.

   ...The only difference is that your paper will be reviewed by an internal committee before submission to ...

10. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...read the titles to find the ones you’d like to revisit. Strategically traverse the sea of posters If you...

11. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    .... The resulting list of hits is then carefully reviewed. As mentioned earlier, it is common to find DNA...

12. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...faring with users in a minute, but, to start, let's review why NgAgo is in the spotlight and take a moment...

13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...exclusively to in vitro cell culture. The user should review the current literature about their preferred model...

14. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...special? A major advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and...

15. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...across regions. Our investigation of model fit revealed some potential instabilities in the China data...

17. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...advantage of being directional, meaning that (in the previous example) one can control whether the third module...

18. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    Published

    July 9, 2019, 12:52 p.m.

    ... my presentation had only become public in the previous two weeks, which demonstrated to the students ...

19. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed ...

20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...