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TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....that it’s never too early to start networking! Learning about careers at Addgene Afterwards, the students...career paths, Michelle Healy said “You are always learning and that means it is possible to switch your field...
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More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog PostPublishedJune 16, 2015, 2:15 p.m.... help save you from performing any unnecessary cloning - the list of "Associated Plasmids" from these ...associated with this "Citing Article" - such as cloning their gene of interest into a previously deposited... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols...purchasing competent cells. However, for most everyday cloning, homemade competent cells will work just fine. ... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....not. In the lab, you could be managing a team, planning meetings, and finding the best way to organize...organize your time. At a conference, you could be honing your science communications skills and networking. ... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14... adjacent CRISPR array and intergenic regions containing the putative tracrRNA into a plasmid and expressed... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids - If you're familiar...they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA ... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here. But the... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed a CRISPR google ... need to get back to emailing (hopefully non-vacationing) scientists. You can find all of our August posts... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m....future, and hopefully include some of our favorite winning recipes. Hack Day lunches These days everyone ...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....identified 1,049 perturbations in several genes spanning 33 cancer types. The types of mutations they identified...targeted therapeutic interventions for cancer. This screening platform is an incredible boost to the cancer ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....labs that already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding...microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....check it out!). After months of strategizing, designing, developing, testing - and repeat - we’re proud... a make-over as well. While in the course of redesigning the homepage, and striving to tell our story ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....large genes, smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To...customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing ... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....Tom Ellis, and George Church for answering our burning questions about how they manage and motivate their...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which differ...334 . https://doi.org/10.1007/s40259-017-0234-5 Büning H, Srivastava A (2019) Capsid Modifications for... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....and annotated from more than 45 publications, beginning with the seminal research articles that demonstrated...Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....whole animal models like Drosophila melanogaster, obtaining enough material for a ChIP-seq experiment can ..., the Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m.... identify the same feature in other plasmids. Aligning sequences with Snapgene Once we’ve identified...plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ...