We narrowed to 695 results for: prot

...factor expected to impact repair is that the Cas9 protein doesn’t immediately release from the broken end... CRISPR 101: Homology Directed Repair CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines ...

...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

... plasma membrane localization of transmembrane proteins, like rhodopsins, are highly constrained and poorly...design new channelrhodopsins with high-throughput proteins engineering methods, like directed evolution, ...

... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...

...require an ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional...

...Check out our AAV Production Protocol Check out our AAV Purification Protocol ...

...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...

...molecularly characterized by misfolding of the TTR protein, which contributes to the disease phenotype. Intellia...showed a high success rate in clearing misfolded protein levels as measured in the blood. More information...

...exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed...response set off by a DREADD depends on what G-protein and, consequently, what pathway the receptor acts...

...enzymatic machinery (i.e. RNA replication with no proteins). The chemistry is complex and this work requires...system. His lab is also exploring the use of cas9 proteins from different species for genome engineering....

... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...multiple PAMs should have a fitness advantage over proteins with single PAM recognition. Created with...

...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

...tissues, eliminating the need to deliver the Cas protein altogether. Platforming AAV Interest in interrogating...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...

...the looping of chromatin, the complex of DNA and proteins that forms chromosomes. These long range DNA interactions... of the key steps of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested...

... can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...

...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002...

... BCA assay uses a serial dilution of a control protein, incubated alongside an unknown sample with the... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, which deaminate...