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TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....the market between 1997 and 2000. There are many reasons why drugs fail - sex is one of them. Why might ...
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Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive charge in... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....career counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....to see if it leads you to the same conclusion. Personally, I like papers that can end with some sort of... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....within E. coli host cells. Image from Nielson and Voigt (2014). We talked with Voigt about... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed PMID: 2839833. PubMed Central... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20 kHz, produced from fluorescent... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m.... in the lab but also causes outbreaks of food poisoning, the difference is virulence plasmids! This category... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....interviewer asks you a smart question. There’s no reason you can’t then ask that same question in your next... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....and really get it.” On the other hand, there are reasons why grad school might not be the right choice. ... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods. MoClo... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....susceptible to artifacts and pH interference, and comparison between experiments can be more difficult due... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter as many search... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....performing these experiments have a good baseline for comparison. Performing WISH using these plasmids can result... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham, R., Benito, R., Boeke... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....properties and can be used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime...