We narrowed to 798 results for: REP

... evolution, Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....

...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

...scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents.... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

... a position as an application support/sales representative in industry or a postdoc in academia, but knew...the week of our interview, Hans was also busy preparing materials for an upcoming product launch. “The...

...world choose to share their plasmids through our repository since we know biological resource centers, including... lack the resources or personnel to constantly prepare them for distribution. One of the major advantages...

...an engineer. The talks we heard Monday night represented engineering first and foremost, from the custom...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...

...systems during experiments – neither ideal for the intrepid researcher. There existed, therefore, a need for...disturbed, the brain fibers could not be directly repurposed for gut implantation. The fibers they ended up...

...with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...) using in utero electroporation or with AAVs (prepared with Addgene #104110 - #104112) (Figure 2). We...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

...swapped for alternative genetic cargo, they can’t reproduce more functional viruses later on. These built-... is! Addgene has a number of ready-to-use viral preps available for a variety of broadly applicable and...

...taking beautiful photographs of their studies, reporting on amazing women in science, or asking someone...yourself open to conversation — put your work on preprint servers, share your email address far and wide...

...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

... the study has just been uploaded to the preprint repository BioRxiv. This example clearly illustrates...

... we found in our map have an impact on virus replication. While we and our collaborators are starting ...straight-forward. All constructs are tagged with a 2xStrep-tag for easy detection and affinity purification...

...ahead of time so that lab members have time to prepare their thoughts. Then at the lab meeting, the team...everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from the desk to...

.... Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target site...this system out on chromosome 19 by targeting 46 repetitive loci that contained  ≥20 copies of the gRNA target...

...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...

...those of the guest blogger and do not necessarily represent the views of Addgene. I think it’s safe to assume...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...

...of simple biological machines R bodies can repeatedly extend and contract in response to changing pH... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...

...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...