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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 777 results for: REP

Showing: 561 - 580 of 777 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...enough that everyone wants to recruit rather than repel and hoard.” If you've already secured a faculty ...Tom Ellis has a one word answer: “Email. Nothing prepared me for what a PI without a personal assistant ...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...(nick, bind, cleave, mixed). This information represents the ‘big picture’ features of the enzymes documented...Classification System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...addition of a helper virus or plasmid allows it to replicate episomally, essentially remaining an extrachromosomal...extrachromosomal part of the host genome that can replicate and divide along with the endogenous DNA. AAV also...

  10. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...which there are never enough, especially in underrepresented groups) reach multiple mentees efficiently...pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or watched during...

  16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...research and how they will use plasmids and viral preps from Addgene to further their work. The Association...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...problem, you might even be expected to write up a report to communicate your findings to your colleagues... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of...

  18. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...their Cell paper, the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of...Biotechnology Find the PRIME-del plasmids  Deletion and replacement of long genomic sequences The PEDAR strategy ...

  20. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...
Showing: 561 - 580 of 777 results