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... communication about experimental methods and approaches. It’s very easy to think that the methods section...increase dramatically. I’m not sure what the best approach to doing that will be, and it will need some effort...

...over several millennia as their paths cross with each other and with the events of the times."     - Aliyah...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

...combative, field. Of course they are competing with each other, but it's a healthy competition. What an honor...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

...this question led us to develop a new, rational approach to improve the efficiency of sequence replacement... order of magnitude greater than transfection approaches. Moreover, the cost of reagents is relatively...

...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...multicomponent assembly have 5′ or 3′ sequence homology to each other, they may be assembled incorrectly. To overcome...

...interactions, cancer immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation... Susanna Bachle...

...tracking the reaction progress as in real time PCR, each reaction partition is individually assessed for ...The amount of both mutated and wild type target in each sample can be quickly calculated (details in [2]...

...slides, learn from audiences as they do science outreach and engage with the public, and learn from the...science communication to more graduate students.   Each June, graduate student leaders in science communication...

...three types of inducible promoters, and examples of each type. Many of these promoter systems are available...promoter. Experimental System Because transcription machinery differs between cell types or organisms, promoters...

...replicate episomally, essentially remaining an extrachromosomal part of the host genome that can replicate...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...helpful in verifying mutations that wouldn't be reached by sequencing from either terminus. Want to know...

...suggestions for using LinkedIn and Facebook. For each of the three social media platforms, there’s some...blog writing tips are elements I try to include in each post I write for the Addgene blog. 5. "Social Media...

...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...lot like a game of telephone, until the message reaches the nucleus where gene expression changes lead ...

...conventional breeding; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

...and effectively presenting the value tailored to each and every audience uniquely (not a focus in graduate... usually) is one of the key skills required to achieve success in this business. A good presentation can...

...important to establish the type of audience you want to reach with your paper. Is it the wider scientific community... and the entire referee report are permanently attached to the published article. In addition, authors...

...if desired, but it's not required for their use. Each RANbody is detectable by two different tags: a reporter...particular applications. Read on to learn more about each reporter or check out table 1 for a summary. HRP...

...There are lots of ways scientists communicate with each other: from formal mechanisms like publishing a ...potential collaborators, blogging and more. These each have different goals, formats, and processes, and...

...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...