Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 816 results for: ache

Showing: 561 - 580 of 816 results

  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...helpful in verifying mutations that wouldn't be reached by sequencing from either terminus. Want to know...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...conventional breeding; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

  4. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...important to establish the type of audience you want to reach with your paper. Is it the wider scientific community... and the entire referee report are permanently attached to the published article. In addition, authors...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...if desired, but it's not required for their use. Each RANbody is detectable by two different tags: a reporter...particular applications. Read on to learn more about each reporter or check out table 1 for a summary. HRP...

  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...There are lots of ways scientists communicate with each other: from formal mechanisms like publishing a ...potential collaborators, blogging and more. These each have different goals, formats, and processes, and...

  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...

  10. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible Cas enzymes Are we truly PAM-less? Have we reached an essentially PAM-free solution? Almost! Assuming...

  11. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...about the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update ...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...infect more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will ...at increasing scale over the course of 1-2 weeks. Each round of amplification should result in a 10-100...

  18. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...share a common genetic background, which means that each mouse is genetically identical and all mice of the...genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the desired genotype...

  19. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...
Showing: 561 - 580 of 816 results