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Showing: 81 - 120 of 155 results

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...decades, but their use can be limited by a few caveats. Since fluorescent proteins (FP) require external...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...are typically injected into brain regions of mice, rats, or non-human primates. To activate the DREADD, ...

  4. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she was fascinated...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...suggestion for CRISPR online course for beginners” on r/labrats, Reddit.  Part of that work meant facilitating ...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... and have similar advantages, though with some caveats.  First off, F(ab’)2 fragments are bivalent, so...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding affinity, can...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...steps keep the cost low compared to other antibody formats. This low barrier to entry makes polyclonal antibodies...

  12. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... by bidirectional transcription or as inverted repeats. These elements are almost always epigenetically...

  13. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature. March 9 2017. ...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer...

  20. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...mind that our customer support scientists wear many hats at this still relatively small nonprofit. If you...

  25. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...then cleaving the ssDNA strands. In this way, Cas3 eats away at both the non-target and target strands by...

  26. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely non-...

  27. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...targeting the viral genome or inverted terminal repeats (ITR). You should always have a high-quality plasmid...

  28. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Hardly! 9E10’s accessibility and the variety of formats you can find it in likely mean it will remain an...

  30. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression systems including their advantages and caveats to keep in mind before choosing a system. Mammalian...

  32. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...that were previously difficult to engineer, like rats. If you have tools for creating knockout animals...

  33. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality...

  35. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...with 3DISCO to image non-transgenic animals such as rats (17).  In addition, the use of viruses allows much...

  38. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit point...
Showing: 81 - 120 of 155 results