We narrowed to 78 results for: 109
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TypeBlog PostPublishedJune 4, 2015, 1:03 p.m.... the lacZΔM15 mutation): XL1-Blue, DH5α, DH10B, JM109, STBL4, JM110, and Top10 are a few examples. Make...
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Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m.... a few popular lab strains, such as NEB Stable, JM109, XL1 Blue or XL10 Gold, that are F’. These strains... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org View Addgene's ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m.... Virol. 2000 Sep;81(Pt 9):2147-53. Pubmed PMID 10950970. 4. Lafon M. Rabies virus receptors. J Neurovirol... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....Nucleic Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar/gkab507 Resources on Addgene.org Addgene... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....https://doi.org/10.1038/s41467-023-38582-7. PMID: 37291094. Recombinant anti-FLAG tag antibodies By Ashley... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m.... Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman J, Vora S, Pruitt... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....Transcription, and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032. ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m.... Duong et al., Genetics 2021. https://doi.org/10.1093/genetics/iyab006 The CRISPR corner By Jennifer... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z.... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....Science 290.5496 (2000): 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m..... Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m.... J Epidemiol, 117(4), 455–466. https://doi.org/10.1093/oxfordjournals.aje.a113563. PMID: 6301263. Servellita... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....EMBO Journal, 16(16), 5113–5122. https://doi.org/10.1093/EMBOJ/16.16.5113. PubMed PMID: 9305652. PubMed ... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....Transactions Royal Soc 380:20210110. https://doi.org/10.1098/rsta.2021.0110 Sherry DM, Stiles MA (2022) Improved... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006) Design and implementation... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m.... the construct. They identified ADAR2DD(delta984-1090), which decreases the REPAIRv1 construct size from... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X., Liu, ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....containing the ADAR2 truncation ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing them...