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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 117 results for: 199

Showing: 41 - 60 of 117 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Committee on Methods of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that ... Washington (DC): National Academies Press (US); 1999. 1, Generation of Hybridomas: Permanent Cell Lines...

  2. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... elute proteins with up to 95% purity (Janknecht 1991, Hochuli 1988). In this purification approach, the...recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, ...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...According to the US General Accounting Office, between 1997 and 2000, ten drugs were withdrawn from the US market...ten drugs were withdrawn from the market between 1997 and 2000. There are many reasons why drugs fail ...

  4. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...Type IIS restriction enzymes, first discovered in 1996. Type IIS restriction enzymes are unique from "traditional...Skowron PM, Rutkowska SM, Hong SS, Kim SC. Genet Anal.1996 Dec;13(6):139-45. PubMed. A one pot, one step, precision...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...transgenic mice. Orban, P.C., Chui, D., and Marth, J.D. 1992. PubMed PMID: 1495975. PubMed Central PMCID: PMC49604...gene targeting. Gu, H., Zou, Y.R., and Rajewsky, K. 1993. PubMed PMID: 8513499. Resources on Addgene.org ...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...responses as decreased heart rate (Redfern et al., 1999) or altered neuron excitability (Zhu et al., 2014...Hennighausen L, Bujard H, Fishman GI, Conklin BR (1999) Conditional expression and signaling of a specifically...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... vitro analyses. Molecular and Cellular Biology. 1992. PMID: 1333042. PubMed Central PMCID: PMC360476....Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: 10074136. PubMed Central PMCID: PMC104046...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...isolated from reef-building corals (Dove et al., 1995). In the early 2000s, a handful of chromoproteins...jbc.m606921200 Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and Partial Characterization of the Pink...

  10. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...enzyme-linked immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (Aurnhammer et al., 2012..., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via a novel capsid...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... – 39.
 A. H. Voie, D. H. Burns, F. A. Spelman. (1993)  J. Microsc. 170, 229 – 236.
 Pubmed. P.A Santi....-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction coefficient...absorption coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. ...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape,...improve its physical and biochemical properties. In 1995, Roger Y. Tsien described an S65T point mutation...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence...abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366. PubMed PMID: 2265611. PubMed Central...

  16. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know that MECP2 binds to methylated CpG...Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused by mutations in X-linked...

  17. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...can have endogenous GUS-like activity (Hu et al., 1990). We commonly use pSB161 - pL2_pSB90_2x35S::GUS:...activities in seed plants." Plant cell reports 9.1 (1990): 1-5. PubMed PMID: 24226366. Lee, Min Woo, and ...

  18. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing viral capsids...production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J....

  19. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...replication in different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control... Doran, K. S., Konieczny, I., & Helinski, D. R. (1998). Replication Origin of the Broad Host Range Plasmid...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular...Unit 5.17. PubMed PMID:18429245. 3. Ptashne, M. (1992). A Genetic Switch: Phage (Lambda) and Higher Organisms...
Showing: 41 - 60 of 117 results